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PMID:18403489

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Citation

Abel, MH, Baker, PJ, Charlton, HM, Monteiro, A, Verhoeven, G, De Gendt, K, Guillou, F and O'Shaughnessy, PJ (2008) Spermatogenesis and sertoli cell activity in mice lacking sertoli cell receptors for follicle-stimulating hormone and androgen. Endocrinology 149:3279-85

Abstract

Spermatogenesis in the adult male depends on the action of FSH and androgen. Ablation of either hormone has deleterious effects on Sertoli cell function and the progression of germ cells through spermatogenesis. In this study we generated mice lacking both FSH receptors (FSHRKO) and androgen receptors on the Sertoli cell (SCARKO) to examine how FSH and androgen combine to regulate Sertoli cell function and spermatogenesis. Sertoli cell number in FSHRKO-SCARKO mice was reduced by about 50% but was not significantly different from FSHRKO mice. In contrast, total germ cell number in FSHRKO-SCARKO mice was reduced to 2% of control mice (and 20% of SCARKO mice) due to a failure to progress beyond early meiosis. Measurement of Sertoli cell-specific transcript levels showed that about a third were independent of hormonal action on the Sertoli cell, whereas others were predominantly androgen dependent or showed redundant control by FSH and androgen. Results show that FSH and androgen act through redundant, additive, and synergistic regulation of spermatogenesis and Sertoli cell activity. In addition, the Sertoli cell retains a significant capacity for activity, which is independent of direct hormonal regulation.

Links

PubMed PMC2592075 Online version:10.1210/en.2008-0086

Keywords

Androgens/pharmacology; Animals; Female; Follicle Stimulating Hormone/pharmacology; Gene Expression Regulation/drug effects; Genotype; Male; Mice; Mice, Knockout; Phenotype; RNA, Messenger/genetics; RNA, Messenger/metabolism; Receptors, Androgen/genetics; Receptors, Androgen/physiology; Receptors, FSH/genetics; Receptors, FSH/physiology; Reverse Transcriptase Polymerase Chain Reaction; Sertoli Cells/drug effects; Sertoli Cells/metabolism; Spermatogenesis/drug effects; Spermatogenesis/genetics; Spermatogenesis/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:ANDR

involved_in

GO:0007283: spermatogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3034098

P

Seeded From UniProt

complete

MOUSE:FSHR

involved_in

GO:0060011: Sertoli cell proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2384087

P

Seeded From UniProt

complete

MOUSE:FSHR

involved_in

GO:0007283: spermatogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2384087

P

Seeded From UniProt

complete

MOUSE:ANDR

acts_upstream_of_or_within

GO:0007283: spermatogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3034098

P

Seeded From UniProt

complete

See also

References

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