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PMID:18369462

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Citation

Li, C, Inglis, PN, Leitch, CC, Efimenko, E, Zaghloul, NA, Mok, CA, Davis, EE, Bialas, NJ, Healey, MP, Héon, E, Zhen, M, Swoboda, P, Katsanis, N and Leroux, MR (2008) An essential role for DYF-11/MIP-T3 in assembling functional intraflagellar transport complexes. PLoS Genet. 4:e1000044

Abstract

MIP-T3 is a human protein found previously to associate with microtubules and the kinesin-interacting neuronal protein DISC1 (Disrupted-in-Schizophrenia 1), but whose cellular function(s) remains unknown. Here we demonstrate that the C. elegans MIP-T3 ortholog DYF-11 is an intraflagellar transport (IFT) protein that plays a critical role in assembling functional kinesin motor-IFT particle complexes. We have cloned a loss of function dyf-11 mutant in which several key components of the IFT machinery, including Kinesin-II, as well as IFT subcomplex A and B proteins, fail to enter ciliary axonemes and/or mislocalize, resulting in compromised ciliary structures and sensory functions, and abnormal lipid accumulation. Analyses in different mutant backgrounds further suggest that DYF-11 functions as a novel component of IFT subcomplex B. Consistent with an evolutionarily conserved cilia-associated role, mammalian MIP-T3 localizes to basal bodies and cilia, and zebrafish mipt3 functions synergistically with the Bardet-Biedl syndrome protein Bbs4 to ensure proper gastrulation, a key cilium- and basal body-dependent developmental process. Our findings therefore implicate MIP-T3 in a previously unknown but critical role in cilium biogenesis and further highlight the emerging role of this organelle in vertebrate development.

Links

PubMed PMC2268012 Online version:10.1371/journal.pgen.1000044

Keywords

Animals; Animals, Genetically Modified; Base Sequence; Biological Transport, Active; Caenorhabditis elegans/genetics; Caenorhabditis elegans/growth & development; Caenorhabditis elegans/physiology; Caenorhabditis elegans Proteins/chemistry; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/physiology; Cilia/physiology; DNA Primers/genetics; DNA, Helminth/genetics; Flagella/physiology; Genes, Helminth; Humans; Intracellular Signaling Peptides and Proteins/chemistry; Intracellular Signaling Peptides and Proteins/genetics; Intracellular Signaling Peptides and Proteins/physiology; Microtubule-Associated Proteins/chemistry; Microtubule-Associated Proteins/genetics; Microtubule-Associated Proteins/physiology; Morphogenesis; Multiprotein Complexes; Mutation; Neurons, Afferent/physiology; Phenotype; Signal Transduction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:Q17595

located_in

GO:0005930: axoneme

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:Q17595

located_in

GO:0036064: ciliary basal body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:Q17595

part_of

GO:0030992: intraciliary transport particle B

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:Q17595

involved_in

GO:0065003: protein-containing complex assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00089143

P

Seeded From UniProt

complete

CAEEL:Q17595

involved_in

GO:0055088: lipid homeostasis

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00089143

P

Seeded From UniProt

complete

CAEEL:Q17595

involved_in

GO:0045184: establishment of protein localization

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00089143

P

Seeded From UniProt

complete

CAEEL:Q17595

involved_in

GO:0042073: intraciliary transport

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00089143

P

Seeded From UniProt

complete

DANRE:MIPT3

involved_in

GO:0060271: cilium assembly

ECO:0000250: sequence similarity evidence used in manual assertion

protein_id:AAC47924

P

Seeded From UniProt

complete


See also

References

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