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PMID:18364354

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Citation

Soh, H and Goldstein, SA (2008) I SA channel complexes include four subunits each of DPP6 and Kv4.2. J. Biol. Chem. 283:15072-7

Abstract

Kv4 potassium channels produce rapidly inactivating currents that regulate excitability of muscles and nerves. To reconstitute the neuronal A-type current I(SA), Kv4 subunits assemble with DPP6, a single transmembrane domain accessory subunit. DPP6 alters function-accelerating activation, inactivation, and recovery from inactivation-and increases surface expression. We sought here to determine the stoichiometry of Kv4 and DPP6 in complexes using functional and biochemical methods. First, wild type channels formed from subunit monomers were compared with channels carrying subunits linked in tandem to enforce 4:4 and 4:2 assemblies (Kv4.2-DPP6 and Kv4.2-Kv4.2-DPP6). Next, channels were overexpressed and purified so that the molar ratio of subunits in complexes could be assessed by direct amino acid analysis. Both biophysical and biochemical methods indicate that I(SA) channels carry four subunits each of Kv4.2 and DPP6.

Links

PubMed PMC2397469 Online version:10.1074/jbc.M706964200

Keywords

Animals; Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; Humans; Multiprotein Complexes/genetics; Multiprotein Complexes/metabolism; Muscles/metabolism; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Neurons/metabolism; Peptide Hydrolases/genetics; Peptide Hydrolases/metabolism; Potassium Channels/genetics; Potassium Channels/metabolism; Protein Structure, Quaternary/physiology; Protein Structure, Tertiary/physiology; Shal Potassium Channels/genetics; Shal Potassium Channels/metabolism; Xenopus laevis

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:DPP6

located_in

GO:0016021: integral component of membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:DPP6

part_of

GO:0008076: voltage-gated potassium channel complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:DPP6

enables

GO:0015459: potassium channel regulator activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:DPP6

part_of

GO:0016021: integral component of membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:DPP6

involved_in

GO:1901379: regulation of potassium ion transmembrane transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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