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PMID:18350154

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Citation

Ryan, PL and Young, JA (2008) Evidence against a human cell-specific role for LRP6 in anthrax toxin entry. PLoS ONE 3:e1817

Abstract

The role of the cellular protein LRP6 in anthrax toxin entry is controversial. Previous studies showed that LRP6 was important for efficient intoxication of human M2182 prostate carcinoma cells but other studies performed with cells from gene-knockout mice demonstrated no role for either LRP6 or the related LRP5 protein in anthrax toxin entry. One possible explanation for this discrepancy is that LRP6 may be important for anthrax toxin entry into human, but not mouse, cells. To test this idea we have investigated the effect of knocking down LRP6 or LRP5 expression with siRNAs in human HeLa cells. We show here that efficient knockdown of either LRP6, LRP5, or both proteins has no influence on the kinetics of anthrax lethal toxin entry or MEK1 substrate cleavage in these cells. These data argue against a human-specific role for LRP6 in anthrax toxin entry and suggest instead that involvement of this protein may be restricted to certain cell types independently of their species of origin.

Links

PubMed PMC2263128 Online version:10.1371/journal.pone.0001817

Keywords

Antigens, Bacterial/toxicity; Bacterial Toxins/toxicity; Base Sequence; HeLa Cells; Humans; LDL-Receptor Related Proteins/physiology; Low Density Lipoprotein Receptor-Related Protein-6; RNA, Small Interfering

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:LRP6

NOT|enables

GO:0019534: toxin transmembrane transporter activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:LRP5

NOT|enables

GO:0019534: toxin transmembrane transporter activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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