GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:18334216

From GONUTS
Jump to: navigation, search
Citation

Koehnke, J, Jin, X, Trbovic, N, Katsamba, PS, Brasch, J, Ahlsen, G, Scheiffele, P, Honig, B, Palmer, AG 3rd and Shapiro, L (2008) Crystal structures of beta-neurexin 1 and beta-neurexin 2 ectodomains and dynamics of splice insertion sequence 4. Structure 16:410-21

Abstract

Presynaptic neurexins (NRXs) bind to postsynaptic neuroligins (NLs) to form Ca(2+)-dependent complexes that bridge neural synapses. beta-NRXs bind NLs through their LNS domains, which contain a single site of alternative splicing (splice site 4) giving rise to two isoforms: +4 and Delta. We present crystal structures of the Delta isoforms of the LNS domains from beta-NRX1 and beta-NRX2, crystallized in the presence of Ca(2+) ions. The Ca(2+)-binding site is disordered in the beta-NRX2 structure, but the 1.7 A beta-NRX1 structure reveals a single Ca(2+) ion, approximately 12 A from the splice insertion site, with one coordinating ligand donated by a glutamic acid from an adjacent beta-NRX1 molecule. NMR studies of beta-NRX1+4 show that the insertion sequence is unstructured, and remains at least partially disordered in complex with NL. These results raise the possibility that beta-NRX insertion sequence 4 may function in roles independent of neuroligin binding.

Links

PubMed PMC2750865 Online version:10.1016/j.str.2007.12.024

Keywords

Amino Acid Sequence; Animals; Calcium/metabolism; Cell Adhesion Molecules, Neuronal; Crystallography, X-Ray; Membrane Proteins/metabolism; Models, Biological; Models, Molecular; Molecular Sequence Data; Nerve Tissue Proteins/chemistry; Nerve Tissue Proteins/metabolism; Protein Binding; Protein Isoforms/chemistry; Protein Structure, Tertiary; RNA Splice Sites; Rats; Recombinant Proteins/chemistry; Sequence Homology, Amino Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:NRX1A

enables

GO:0005509: calcium ion binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

RAT:NRX1B

enables

GO:0005509: calcium ion binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.