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PMID:18316477

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Citation

Wen, H, Andrejka, L, Ashton, J, Karess, R and Lipsick, JS (2008) Epigenetic regulation of gene expression by Drosophila Myb and E2F2-RBF via the Myb-MuvB/dREAM complex. Genes Dev. 22:601-14

Abstract

The Drosophila Myb oncoprotein, the E2F2 transcriptional repressor, and the RBF and Mip130/LIN-9 tumor suppressor proteins reside in a conserved Myb-MuvB (MMB)/dREAM complex. We now show that Myb is required in vivo for the expression of Polo kinase and components of the spindle assembly checkpoint (SAC). Surprisingly, the highly conserved DNA-binding domain was not essential for assembly of Myb into MMB/dREAM, for transcriptional regulation in vivo, or for rescue of Myb-null mutants to adult viability. E2F2, RBF, and Mip130/LIN-9 acted in opposition to Myb by repressing the expression of Polo and SAC genes in vivo. Remarkably, the absence of both Myb and Mip130, or of both Myb and E2F2, caused variegated expression in which high or low levels of Polo were stably inherited through successive cell divisions in imaginal wing discs. Restoration of Myb resulted in a uniformly high level of Polo expression similar to that seen in wild-type tissue, whereas restoration of Mip130 or E2F2 extinguished Polo expression. Our results demonstrate epigenetic regulation of gene expression by Myb, Mip130/LIN-9, and E2F2-RBF in vivo, and also provide an explanation for the ability of Mip130-null mutants to rescue the lethality of Myb-null mutants in vivo.

Links

PubMed PMC2259030 Online version:10.1101/gad.1626308

Keywords

Animals; Carrier Proteins/genetics; Carrier Proteins/metabolism; Caspases/metabolism; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/genetics; E2F2 Transcription Factor/genetics; E2F2 Transcription Factor/metabolism; Epigenesis, Genetic; Green Fluorescent Proteins/analysis; Green Fluorescent Proteins/genetics; Mitosis/genetics; Neoplasms/genetics; Protein-Serine-Threonine Kinases/genetics; Proto-Oncogene Proteins c-myb/genetics; Proto-Oncogene Proteins c-myb/metabolism; Retinoblastoma Protein/genetics; Retinoblastoma Protein/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:MYB

involved_in

GO:0000278: mitotic cell cycle

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:MYB

involved_in

GO:0007088: regulation of mitotic nuclear division

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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