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PMID:18281333

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Citation

Southon, A, Farlow, A, Norgate, M, Burke, R and Camakaris, J (2008) Malvolio is a copper transporter in Drosophila melanogaster. J. Exp. Biol. 211:709-16

Abstract

Divalent metal ion transporter 1 (DMT1; also known as SLC11A2) can transport several metals including Fe and Cu in mammalian systems. We set out to determine whether Malvolio (Mvl), the Drosophila melanogaster orthologue of DMT1, can also transport Cu. Overexpression of Mvl caused Cu accumulation in Drosophila S2 cultured cells and conversely dsRNAi knockdown of endogenous Mvl reduced cellular Cu levels. Cell viability under Cu limiting conditions was reduced following dsRNAi knockdown. A homozygous viable Mvl loss-of-function mutant (Mvl(97f)) was sensitive to excess Cu and female Mvl(97f) flies were also sensitive to Cu limitation. An MtnA-EYFP reporter was used as a proxy measure of Cu distribution within Mvl(97f/+) larvae. Under basal conditions Cu levels were reduced in the anterior midgut and proventriculus relative to control larvae. These results demonstrate Mvl is a functional Cu transporter and that despite partial functional redundancy with the Ctr1 proteins, Cu uptake through this pathway is necessary for optimal viability at the cellular and organismal levels.

Links

PubMed Online version:10.1242/jeb.014159

Keywords

Animals; Biological Transport/physiology; Cell Survival/genetics; Cell Survival/physiology; Cells, Cultured; Copper/metabolism; DNA Primers/genetics; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/genetics; Drosophila melanogaster/metabolism; Drosophila melanogaster/physiology; Female; Ion Pumps/genetics; Ion Pumps/metabolism; Larva/growth & development; Larva/metabolism; Male; Membrane Proteins/genetics; Membrane Proteins/metabolism; Microscopy, Fluorescence; Pigmentation/genetics; Pigmentation/physiology; RNA Interference

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:MVL

enables

GO:0005375: copper ion transmembrane transporter activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:MVL

involved_in

GO:0015677: copper ion import

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:MVL

located_in

GO:0005768: endosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:MVL

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:MVL

involved_in

GO:0055070: copper ion homeostasis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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