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PMID:18276122

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Citation

Ohno, K, McCabe, D, Czermin, B, Imhof, A and Pirrotta, V ESC, ESCL and their roles in Polycomb Group mechanisms. Mech. Dev. 125:527-41

Abstract

The Drosophila esc gene is a Polycomb Group (PcG) gene whose product is essential for histone H3 K27 methylation and PcG silencing yet genetic analysis indicated that its product was needed only in the very early embryo. We know now that escl, a close homologue of esc exists in the Drosophila genome. In contrast with earlier studies, we find that both esc and escl are expressed at all stages of development. We show that three major differences between the two genes are in the transcriptional control, which allows esc to make a much stronger maternal contribution; in the splicing efficiency, which makes a major difference in the early escl function; and in the lower participation of ESCL in the PRC2 complex and lower enzymatic activity of the resulting complex. Both genes can sustain normal development in the absence of the other except for the critical role provided by maternal esc product in early embryonic development. Finally, using zygotic mutations in both genes, we show that the gradual loss of function of PRC2 activity leads first to a loss of histone H3 K27 methylation and only at a later stage to a gradual loss of PRC1 binding to chromatin.

Links

PubMed Online version:10.1016/j.mod.2008.01.002

Keywords

Alleles; Alternative Splicing; Animals; Binding, Competitive; DNA Methylation; Drosophila Proteins/genetics; Drosophila Proteins/physiology; Drosophila melanogaster/metabolism; Gene Deletion; Gene Expression Regulation, Developmental; Histone-Lysine N-Methyltransferase/metabolism; Histones/metabolism; Homeodomain Proteins/genetics; Models, Genetic; Protein Methyltransferases; Proteins/metabolism; RNA Interference; RNA-Binding Proteins; Repressor Proteins/genetics; Repressor Proteins/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:EZ

enables

GO:0042054: histone methyltransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:EZ

part_of

GO:0035098: ESC/E(Z) complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:EZ

involved_in

GO:0034968: histone lysine methylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:ESC

part_of

GO:0035098: ESC/E(Z) complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:ESC

involved_in

GO:0070734: histone H3-K27 methylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:CAF1

part_of

GO:0035098: ESC/E(Z) complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:CAF1

involved_in

GO:0034968: histone lysine methylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q95SW6

part_of

GO:0035098: ESC/E(Z) complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q95SW6

involved_in

GO:0070734: histone H3-K27 methylation

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0000588

P

Seeded From UniProt

complete

DROME:SUZ12

part_of

GO:0035098: ESC/E(Z) complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:SUZ12

involved_in

GO:0034968: histone lysine methylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VKD5

part_of

GO:0035098: ESC/E(Z) complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VKD5

involved_in

GO:0070734: histone H3-K27 methylation

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0000588

P

Seeded From UniProt

complete


See also

References

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