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PMID:18258486

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Citation

Wu, S, Liu, Y, Zheng, Y, Dong, J and Pan, D (2008) The TEAD/TEF family protein Scalloped mediates transcriptional output of the Hippo growth-regulatory pathway. Dev. Cell 14:388-98

Abstract

The Hippo (Hpo) kinase cascade restricts tissue growth by inactivating the transcriptional coactivator Yorkie (Yki), which regulates the expression of target genes such as the cell death inhibitor diap1 by unknown mechanisms. Here we identify the TEAD/TEF family protein Scalloped (Sd) as a DNA-binding transcription factor that partners with Yki to mediate the transcriptional output of the Hpo growth-regulatory pathway. The diap1 (th) locus harbors a minimal Sd-binding Hpo Responsive Element (HRE) that mediates transcriptional regulation by the Hpo pathway. Sd binds directly to Yki, and a Yki missense mutation that abrogates Sd-Yki binding also inactivates Yki function in vivo. We further demonstrate that sd is required for yki-induced tissue overgrowth and target gene expression, and that sd activity is conserved in its mammalian homolog. Our results uncover a heretofore missing link in the Hpo signaling pathway and provide a glimpse of the molecular events on a Hpo-responsive enhancer element.

Links

PubMed Online version:10.1016/j.devcel.2008.01.007

Keywords

Amino Acid Sequence; Animals; Animals, Genetically Modified; Base Sequence; Binding Sites/genetics; DNA/genetics; DNA/metabolism; Drosophila/genetics; Drosophila/growth & development; Drosophila/physiology; Drosophila Proteins/genetics; Drosophila Proteins/physiology; Epistasis, Genetic; Gene Dosage; Gene Expression Regulation, Developmental; Genes, Insect; Inhibitor of Apoptosis Proteins/genetics; Intracellular Signaling Peptides and Proteins/genetics; Intracellular Signaling Peptides and Proteins/physiology; Models, Biological; Molecular Sequence Data; Mutation; Nuclear Proteins/genetics; Nuclear Proteins/physiology; Organ Size/physiology; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/physiology; Sequence Homology, Amino Acid; Signal Transduction; Trans-Activators/genetics; Trans-Activators/physiology; Transcription Factors/genetics; Transcription Factors/physiology; Transcription, Genetic; Wing/growth & development

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:SCAL

enables

GO:0000976: transcription cis-regulatory region binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:YAP1

involved_in

GO:0035329: hippo signaling

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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