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PMID:18250318

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Citation

Zmojdzian, M, Da Ponte, JP and Jagla, K (2008) Cellular components and signals required for the cardiac outflow tract assembly in Drosophila. Proc. Natl. Acad. Sci. U.S.A. 105:2475-80

Abstract

Specification of cardiac primordia and formation of the Drosophila heart tube is highly reminiscent of the early steps of vertebrate heart development. We previously reported that the final morphogenesis of the Drosophila heart involves a group of nonmesodermal cells called heart-anchoring cells and a pair of derived from the pharyngeal mesoderm cardiac outflow muscles. Like the vertebrate cardiac neural crest cells, heart-anchoring cells migrate, interact with the tip of the heart, and participate in shaping the cardiac outflow tract. To better understand this process, we performed an in-depth analysis of how the Drosophila outflow tract is formed. We found that the most anterior cardioblasts that form a central outflow tract component, the funnel-shaped heart tip, do not originate from the cardiac primordium. They are initially associated with the pharyngeal cardiac outflow muscles and join the anterior aorta during outflow tract assembly. The particular morphology of the heart tip is disrupted in embryos in which heart-anchoring cells were ablated, revealing their critical role in outflow tract morphogenesis. We also demonstrate that Slit and Robo are required for directed movements of heart-anchoring cells toward the heart tip and that the cell-cell contact between the heart-anchoring cells and the ladybird-expressing cardioblasts is critically dependent on DE-cadherin Shotgun. Our observations suggest that the similarities between Drosophila and vertebrate cardiogenesis extend beyond the early developmental events.

Links

PubMed PMC2268161 Online version:10.1073/pnas.0706402105

Keywords

Animals; Cadherins/genetics; Cadherins/metabolism; Cell Movement; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/cytology; Drosophila melanogaster/embryology; Drosophila melanogaster/genetics; Drosophila melanogaster/metabolism; Gene Expression Regulation, Developmental; Heart/embryology; Mutation/genetics; Myocardium/cytology; Myocardium/metabolism; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Phenotype; Receptors, Immunologic/genetics; Receptors, Immunologic/metabolism; Signal Transduction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:M9PC46

involved_in

GO:0003151: outflow tract morphogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0005631

P

Seeded From UniProt

complete

DROME:O44924

involved_in

GO:0003151: outflow tract morphogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0002543

P

Seeded From UniProt

complete

DROME:CADE

involved_in

GO:0003151: outflow tract morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q5U176

involved_in

GO:0003151: outflow tract morphogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0005631

P

Seeded From UniProt

complete

DROME:Q9GPP7

involved_in

GO:0003151: outflow tract morphogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0005631

P

Seeded From UniProt

complete

DROME:Q9VQ08

involved_in

GO:0003151: outflow tract morphogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0005631

P

Seeded From UniProt

complete

DROME:Q9W213

involved_in

GO:0003151: outflow tract morphogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0002543

P

Seeded From UniProt

complete

DROME:SLIT

involved_in

GO:0003151: outflow tract morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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