GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:18243098

From GONUTS
Jump to: navigation, search
Citation

Shimada, M, Niida, H, Zineldeen, DH, Tagami, H, Tanaka, M, Saito, H and Nakanishi, M (2008) Chk1 is a histone H3 threonine 11 kinase that regulates DNA damage-induced transcriptional repression. Cell 132:221-32

Abstract

DNA damage results in activation or suppression of transcription of a large number of genes. Transcriptional activation has been well characterized in the context of sequence-specific DNA-bound activators, whereas mechanisms of transcriptional suppression are largely unexplored. We show here that DNA damage rapidly reduces histone H3 Threonine 11 (T11) phosphorylation. This correlates with repression of genes, including cyclin B1 and cdk1. H3-T11 phosphorylation occurs throughout the cell cycle and is Chk1 dependent in vivo. Following DNA damage, Chk1 undergoes rapid chromatin dissociation, concomitant with reduced H3-T11 phosphorylation. Furthermore, we find that loss of H3-T11 phosphorylation correlates with reduced binding of the histone acetyltransferase GCN5 at cyclin B1 and cdk1 promoters and reduced H3-K9 acetylation. We propose a mechanism for Chk1 as a histone kinase, responsible for DNA-damage-induced transcriptional repression by loss of histone acetylation.

Links

PubMed Online version:10.1016/j.cell.2007.12.013

Keywords

Adenoviridae/genetics; Animals; Cells, Cultured; Culture Media, Serum-Free; DNA Damage; Embryo, Mammalian; Fibroblasts/metabolism; Fibroblasts/radiation effects; Gene Expression Regulation; HCT116 Cells; Histones/genetics; Histones/metabolism; Humans; Mice; Models, Genetic; Phosphorylation/radiation effects; Protein Kinases/analysis; Protein Kinases/chemistry; Protein Kinases/genetics; Protein Kinases/metabolism; Protein Kinases/physiology; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/metabolism; Substrate Specificity; Transcription, Genetic; Ultraviolet Rays

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:CHK1

enables

GO:0035402: histone kinase activity (H3-T11 specific)

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:CHK1

part_of

GO:0000785: chromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:CHK1

involved_in

GO:2000615: regulation of histone H3-K9 acetylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CHK1

involved_in

GO:0048096: chromatin-mediated maintenance of transcription

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CHK1

involved_in

GO:0010767: regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CHK1

enables

GO:0035402: histone kinase activity (H3-T11 specific)

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.