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PMID:18202452

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Citation

Schaloske, RH, Blaesius, D, Schlatterer, C and Lusche, DF (2007) Arachidonic acid is a chemoattractant for Dictyostelium discoideum cells. J. Biosci. 32:1281-9

Abstract

Cyclic AMP (cAMP)is a natural chemoattractant of the social amoeba Dictyostelium discoideum. It is detected by cell surface cAMP receptors. Besides a signalling cascade involving phosphatidylinositol 3,4,5-trisphosphate (PIP3), Ca2+ signalling has been shown to have a major role in chemotaxis. Previously, we have shown that arachidonic acid (AA) induces an increase in the cytosolic Ca2+ concentration by causing the release of Ca2+ from intracellular stores and activating influx of extracellular Ca2+. Here we report that AA is a chemoattractant for D. discoideum cells differentiated for 8-9 h. Motility towards a glass capillary filled with an AA solution was dose-dependent and qualitatively comparable to cAMP-induced chemotaxis. Ca2+ played an important role in AA chemotaxis of wild-type Ax2 as ethyleneglycol-bis(b-aminoethyl)-N,N,N',N'-tetraacetic acid (EGTA) added to the extracellular buffer strongly inhibited motility. In the HM1049 mutant whose iplA gene encoding a putative Ins(1,4,5)P3 -receptor had been knocked out, chemotaxis was only slightly affected by EGTA. Chemotaxis in the presence of extracellular Ca2+ was similar in both strains. Unlike cAMP, addition of AA to a cell suspension did not change cAMP or cGMP levels. A model for AA chemotaxis based on the findings in this and previous work is presented.

Links

PubMed

Keywords

Animals; Arachidonic Acid/pharmacology; Calcium/metabolism; Chemotaxis/drug effects; Cyclic AMP/metabolism; Cyclic GMP/metabolism; Dictyostelium/cytology; Dictyostelium/drug effects; Dictyostelium/genetics; Dictyostelium/metabolism; Egtazic Acid/pharmacology; Mutation

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DICDI:IPLA

acts_upstream_of_or_within

GO:0034670: chemotaxis to arachidonic acid

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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