GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:18198337

From GONUTS
Jump to: navigation, search
Citation

Tsai, CJ, Mets, DG, Albrecht, MR, Nix, P, Chan, A and Meyer, BJ (2008) Meiotic crossover number and distribution are regulated by a dosage compensation protein that resembles a condensin subunit. Genes Dev. 22:194-211

Abstract

Biological processes that function chromosome-wide are not well understood. Here, we show that the Caenorhabditis elegans protein DPY-28 controls two such processes, X-chromosome dosage compensation in somatic cells and meiotic crossover number and distribution in germ cells. DPY-28 resembles a subunit of condensin, a conserved complex required for chromosome compaction and segregation. In the soma, DPY-28 associates with the dosage compensation complex on hermaphrodite X chromosomes to repress transcript levels. In the germline, DPY-28 restricts crossovers. In many organisms, one crossover decreases the likelihood of another crossover nearby, an enigmatic process called crossover interference. In C. elegans, interference is complete: Only one crossover occurs per homolog pair. dpy-28 mutations increase crossovers, disrupt crossover interference, and alter crossover distribution. Early recombination intermediates (RAD-51 foci) increase concomitantly, suggesting that DPY-28 acts to limit double-strand breaks (DSBs). Reinforcing this view, dpy-28 mutations partially restore DSBs in mutants lacking HIM-17, a chromatin-associated protein required for DSB formation. Our work further links dosage compensation to condensin and establishes a new role for condensin components in regulating crossover number and distribution. We propose that both processes utilize a related mechanism involving changes in higher-order chromosome structure to achieve chromosome-wide effects.

Links

PubMed PMC2192754 Online version:10.1101/gad.1618508

Keywords

Adenosine Triphosphatases/chemistry; Adenosine Triphosphatases/genetics; Animals; Caenorhabditis elegans; Caenorhabditis elegans Proteins/physiology; Cell Cycle Proteins/physiology; Chromosome Breakage; Crossing Over, Genetic; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/genetics; DNA-Binding Proteins/physiology; Disorders of Sex Development; Dosage Compensation, Genetic; Epigenesis, Genetic; Meiosis; Multiprotein Complexes/chemistry; Multiprotein Complexes/genetics; Mutation; X Chromosome

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:DPY26

part_of

GO:0046536: dosage compensation complex

ECO:0000353: physical interaction evidence used in manual assertion

WB:WBGene00001086
WB:WBGene00001087
WB:WBGene00003367

C

Seeded From UniProt

complete

CAEEL:DPY27

part_of

GO:0046536: dosage compensation complex

ECO:0000353: physical interaction evidence used in manual assertion

WB:WBGene00001085
WB:WBGene00001087
WB:WBGene00003367

C

Seeded From UniProt

complete

CAEEL:MIX1

part_of

GO:0046536: dosage compensation complex

ECO:0000353: physical interaction evidence used in manual assertion

WB:WBGene00001085
WB:WBGene00001086
WB:WBGene00001087

C

Seeded From UniProt

complete

CAEEL:CND1

part_of

GO:0005654: nucleoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:CND1

part_of

GO:0043073: germ cell nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:CND1

part_of

GO:0000794: condensed nuclear chromosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:CND1

part_of

GO:0000805: X chromosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:CND1

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:CND1

part_of

GO:0046536: dosage compensation complex

ECO:0000353: physical interaction evidence used in manual assertion

WB:WBGene00001085
WB:WBGene00001086
WB:WBGene00003367

C

Seeded From UniProt

complete

CAEEL:CND1

involved_in

GO:0007059: chromosome segregation

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00241898
WB:WBVar00275343
WB:WBVar00275349

P

Seeded From UniProt

complete

CAEEL:CND1

involved_in

GO:0045128: negative regulation of reciprocal meiotic recombination

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00241898
WB:WBVar00275343
WB:WBVar00275344
WB:WBVar00275349

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.