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PMID:18187449
| Citation |
Zhang, H, Skop, AR and White, JG (2008) Src and Wnt signaling regulate dynactin accumulation to the P2-EMS cell border in C. elegans embryos. J. Cell. Sci. 121:155-61 |
|---|---|
| Abstract |
In many organisms, the dynein-dynactin complex is required for the alignment of the mitotic spindle onto the axis of polarity of a cell undergoing asymmetric cell division. How this complex transduces polarity cues, either intrinsic or extrinsic, and rotationally aligns the spindle accordingly is not well understood. The Caenorhabditis elegans blastomere P2 polarizes the neighboring EMS blastomere, which causes the EMS spindle to rotationally align along the defined axis of polarity via two redundant signaling pathways: Wnt and Src. Here, we describe how components of the dynactin complex became locally enriched at the P2-EMS border prior to and during rotational alignment of their spindles. Wnt and Src signaling were required for both localized dynactin enrichment, and for rotational alignment of the P2 and EMS spindles. Depleting the trimeric G-protein subunit G alpha did not abolish dynactin accumulation to the P2-EMS border, yet both EMS and P2 spindles failed to rotationally align, indicating that G alpha might act to regulate dynein/dynactin motor activity. By RNAi of a weak dnc-1(ts) allele, we showed that dynactin activity was required at least for EMS spindle rotational alignment. |
| Links |
PubMed Online version:10.1242/jcs.015966 |
| Keywords |
Alleles; Animals; Caenorhabditis elegans; Caenorhabditis elegans Proteins/physiology; Epithelium/metabolism; Gene Expression Regulation; Gene Expression Regulation, Developmental; Green Fluorescent Proteins/metabolism; Mesoderm/metabolism; Microtubule-Associated Proteins/biosynthesis; Microtubule-Associated Proteins/metabolism; Mitotic Spindle Apparatus/metabolism; Models, Biological; Signal Transduction; Wnt Proteins/metabolism; src-Family Kinases/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
CAEEL:E2JKY8 |
located_in |
GO:0005813: centrosome |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
CAEEL:E2JKY8 |
located_in |
GO:0035371: microtubule plus-end |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
CAEEL:E2JKY8 |
located_in |
GO:0005938: cell cortex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
CAEEL:E2JKZ0 |
located_in |
GO:0005813: centrosome |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
CAEEL:E2JKZ0 |
located_in |
GO:0035371: microtubule plus-end |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
CAEEL:E2JKZ0 |
located_in |
GO:0005938: cell cortex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
CAEEL:Q23542 |
located_in |
GO:0005813: centrosome |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
CAEEL:Q23542 |
located_in |
GO:0035371: microtubule plus-end |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
CAEEL:Q23542 |
located_in |
GO:0005938: cell cortex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
See also
References
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