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PMID:18178267

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Citation

Pilar, AV, Madrid, KP and Jardim, A (2008) Interaction of Leishmania PTS2 receptor peroxin 7 with the glycosomal protein import machinery. Mol. Biochem. Parasitol. 158:72-81

Abstract

Leishmania proteins containing a peroxisomal targeting signal sequence 2 (PTS2) are selectively trafficked to the glycosome by associating with the peroxin 7 receptor protein (PEX7). The L. major PEX7 (LmPEX7) encodes a approximately 41 kDa protein that exhibits limited sequence identity with PEX7 homologues from other eukaryotic organisms. Functional characterization of recombinant and native LmPEX7 revealed that this receptor bound the PTS2 protein fructose-1,6-bisphosphate aldolase. Moreover, LmPEX7 also formed a tight association with the Leishmania PEX5, the cytosolic PTS1 receptor, and PEX14, a glycosomal peripheral membrane protein required for protein import into the glycosome. Mapping studies revealed that the Leishmania PEX7 binds to a domain on LdPEX5 encompassing residues 111-148 and to a site on LdPEX14 spanning residues 120-148. Finally, subcellular localization studies revealed that Leishmania PEX7 has a dual distribution within the cytosolic compartment and glycosomal lumen.

Links

PubMed Online version:10.1016/j.molbiopara.2007.11.013

Keywords

Animals; Cytoplasm/chemistry; Fructose-Bisphosphate Aldolase/metabolism; Leishmania major/metabolism; Microbodies/chemistry; Microscopy, Confocal; Models, Biological; Protein Binding; Protein Interaction Mapping; Protozoan Proteins/metabolism; Receptors, Cytoplasmic and Nuclear/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

LEIMA:E9AEW5

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

GeneDB:LmjF.29.0740

F

Seeded From UniProt

complete

LEIMA:Q4QBZ9

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

GeneDB:LmjF.29.0740

F

Seeded From UniProt

complete

LEIMA:Q4VQ66

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

GeneDB:LmjF.35.1420

F

Seeded From UniProt

complete

LEIMA:Q4VQ66

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

LEIMA:Q4VQ66

acts_upstream_of_or_within

GO:0006625: protein targeting to peroxisome

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

LEIMA:Q4VQ66

located_in

GO:0020015: glycosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

LEIMA:Q4VQ66

enables

GO:0005053: peroxisome matrix targeting signal-2 binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

LEIMA:Q4VQ66

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

LEIMA:Q4VQ66

involved_in

GO:0006625: protein targeting to peroxisome

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

LEIMA:Q4VQ66

part_of

GO:0020015: glycosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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