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PMID:18166153

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Citation

Cho, SW, Her, SJ, Sun, HJ, Choi, OK, Yang, JY, Kim, SW, Kim, SY and Shin, CS (2008) Differential effects of secreted frizzled-related proteins (sFRPs) on osteoblastic differentiation of mouse mesenchymal cells and apoptosis of osteoblasts. Biochem. Biophys. Res. Commun. 367:399-405

Abstract

Secreted frizzled-related proteins (sFRPs) are modulators of Wnt signaling. This study was undertaken for definitive assessment of contribution of different sFRPs in osteoblastic differentiation of mesenchymal progenitor cells and apoptosis of osteoblasts. Treatment of C3H10T1/2 cells with sFRP-2 at concentrations of 10, 50, and 100nM and sFRP-4 at low concentrations (5nM) significantly increased Wnt-3A-induced alkaline phosphatase (ALP) activities, whereas sFRP-1 or 3 did not. Retroviral transduction of the sFRP-2 but not other sFRPs also significantly enhanced ALP activity induced by beta-glycerophosphate and ascorbic acid. Furthermore, transfection of all the sFRP expression vectors significantly increased beta-catenin/TCF reporter activity and the effects were most prominent with sFRP-2 and -4. In osteoblast apoptosis assay, only sFRP-3 increased etoposide-induced apoptosis in MC3T3-E1 mouse osteoblasts. In conclusion, we found that different repertoires of sFRPs exert differential effects on osteoblastic differentiation of mouse mesenchymal cells and cellular apoptosis of mouse osteoblasts in vitro.

Links

PubMed Online version:10.1016/j.bbrc.2007.12.128

Keywords

Animals; Apoptosis/physiology; Cell Differentiation/physiology; Cell Line; Glycoproteins/metabolism; Membrane Proteins/metabolism; Mesenchymal Stem Cells/cytology; Mesenchymal Stem Cells/physiology; Mice; Mice, Inbred C3H; Osteoblasts/cytology; Osteoblasts/physiology; Proto-Oncogene Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:DKK1

acts_upstream_of_or_within

GO:0090090: negative regulation of canonical Wnt signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P27467

P

Seeded From UniProt

complete

HUMAN:SFRP4

acts_upstream_of_or_within

GO:0090263: positive regulation of canonical Wnt signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P27467

P

Seeded From UniProt

complete

HUMAN:SFRP3

acts_upstream_of_or_within

GO:0043065: positive regulation of apoptotic process

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P27467

P

Seeded From UniProt

complete

HUMAN:DKK1

involved_in

GO:0090090: negative regulation of canonical Wnt signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P27467

P

Seeded From UniProt

complete

MOUSE:SFRP2

acts_upstream_of_or_within

GO:0045669: positive regulation of osteoblast differentiation

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:98956

P

Seeded From UniProt

complete

MOUSE:SFRP2

acts_upstream_of_or_within

GO:0090263: positive regulation of canonical Wnt signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:98956

P

Seeded From UniProt

complete

HUMAN:SFRP4

involved_in

GO:0090263: positive regulation of canonical Wnt signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P27467

P

Seeded From UniProt

complete

HUMAN:SFRP3

involved_in

GO:0043065: positive regulation of apoptotic process

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P27467

P

Seeded From UniProt

complete


See also

References

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