GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:18095365

From GONUTS
Jump to: navigation, search
Citation

Dihal, AA, van der Woude, H, Hendriksen, PJ, Charif, H, Dekker, LJ, Ijsselstijn, L, de Boer, VC, Alink, GM, Burgers, PC, Rietjens, IM, Woutersen, RA and Stierum, RH (2008) Transcriptome and proteome profiling of colon mucosa from quercetin fed F344 rats point to tumor preventive mechanisms, increased mitochondrial fatty acid degradation and decreased glycolysis. Proteomics 8:45-61

Abstract

Quercetin has been shown to act as an anticarcinogen in experimental colorectal cancer (CRC). The aim of the present study was to characterize transcriptome and proteome changes occurring in the distal colon mucosa of rats supplemented with 10 g quercetin/kg diet for 11 wk. Transcriptome data analyzed with Gene Set Enrichment Analysis showed that quercetin significantly downregulated the potentially oncogenic mitogen-activated protein kinase (Mapk) pathway. In addition, quercetin enhanced expression of tumor suppressor genes, including Pten, Tp53, and Msh2, and of cell cycle inhibitors, including Mutyh. Furthermore, dietary quercetin enhanced genes involved in phase I and II metabolism, including Fmo5, Ephx1, Ephx2, and Gpx2. Quercetin increased PPARalpha target genes, and concomitantly enhanced expression of genes involved in mitochondrial fatty acid (FA) degradation. Proteomics performed in the same samples revealed 33 affected proteins, of which four glycolysis enzymes and three heat shock proteins were decreased. A proteome-transcriptome comparison showed a low correlation, but both pointed out toward altered energy metabolism. In conclusion, transcriptomics combined with proteomics showed that dietary quercetin evoked changes contrary to those found in colorectal carcinogenesis. These tumor-protective mechanisms were associated with a shift in energy production pathways, pointing at decreased cytoplasmic glycolysis and toward increased mitochondrial FA degradation.

Links

PubMed Online version:10.1002/pmic.200700364

Keywords

Animals; Colon/metabolism; Colorectal Neoplasms/metabolism; Colorectal Neoplasms/prevention & control; Diet; Down-Regulation/physiology; Fatty Acids/metabolism; Gene Expression Profiling; Glycolysis/physiology; Intestinal Mucosa/chemistry; Intestinal Mucosa/metabolism; Male; Mitochondria/chemistry; Mitochondria/metabolism; Proteome/metabolism; Quercetin/administration & dosage; Rats; Rats, Inbred F344

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:O54857

involved_in

GO:0014070: response to organic cyclic compound

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:MSH2

involved_in

GO:0014070: response to organic cyclic compound

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.