GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:18094021

From GONUTS
Jump to: navigation, search
Citation

Tian, AG and Deng, WM (2008) Lgl and its phosphorylation by aPKC regulate oocyte polarity formation in Drosophila. Development 135:463-71

Abstract

Specification of the anteroposterior (AP) axis in Drosophila oocytes requires proper organization of the microtubule and actin cytoskeleton. The establishment and regulation of cytoskeletal polarity remain poorly understood, however. Here, we show important roles for the tumor suppressor Lethal (2) giant larvae (Lgl) and atypical protein kinase C (aPKC) in regulating microtubule polarity and setting up the AP axis of the oocyte. Lgl in the germline cells regulates the localization of axis-specifying morphogens. aPKC phosphorylation of Lgl restricts Lgl activity to the oocyte posterior, thereby dividing the cortex into different domains along the AP axis. Active Lgl promotes the formation of actin-rich projections at the oocyte cortex and the posterior enrichment of the serine/threonine kinase Par-1, a key step for oocyte polarization. Our studies suggest that Lgl and its phosphorylation by aPKC may form a conserved regulatory circuitry in polarization of various cell types.

Links

PubMed PMC2753475 Online version:10.1242/dev.016253

Keywords

Actins/metabolism; Animals; Cell Differentiation; Cell Lineage; Cell Polarity; Clone Cells; Drosophila Proteins/metabolism; Drosophila melanogaster/cytology; Drosophila melanogaster/enzymology; Oocytes/cytology; Oocytes/enzymology; Phosphorylation; Protein Binding; Protein Kinase C/metabolism; Protein Transport; Protein-Serine-Threonine Kinases/metabolism; Tumor Suppressor Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:APKC

involved_in

GO:0007314: oocyte anterior/posterior axis specification

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0002121

P

Seeded From UniProt

complete

DROME:L2GL

involved_in

GO:0030036: actin cytoskeleton organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:L2GL

involved_in

GO:0016325: oocyte microtubule cytoskeleton organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:L2GL

involved_in

GO:0007314: oocyte anterior/posterior axis specification

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.