GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:18089289

From GONUTS
Jump to: navigation, search
Citation

Lolicato, F, Marino, R, Paronetto, MP, Pellegrini, M, Dolci, S, Geremia, R and Grimaldi, P (2008) Potential role of Nanos3 in maintaining the undifferentiated spermatogonia population. Dev. Biol. 313:725-38

Abstract

Nanos gene encodes for zinc-finger protein with putative RNA-binding activity which shows an evolutionary conserved function in germ cell development. In the mouse, three Nanos homologs have been identified: Nanos1, Nanos2 and Nanos3. The Nanos3 ortholog is expressed in both male and female gonads of early embryo and, after birth, it is found only in the testis. Nanos3 targeted disruption results in the complete loss of germ cells in both sexes; however the role of Nanos3 in the testis during the postnatal period has not been explored yet. In this study, we show that, in prepuberal testis, Nanos3 is expressed in undifferentiated spermatogonia and that its up-regulation causes accumulation of cells in the G1 phase, indicating that this protein is able to delay the cell cycle progression of spermatogonial cells. This is in line with the observation that the cell cycle length of the undifferentiated germ cells is longer than in differentiating spermatogonia. We also demonstrate a conserved mechanism of action of Nanos3, involving the interaction with the murine RNA-binding protein Pumilio2 and consisting of a potential translational repressor activity. According to the possible role of Nanos3 in inhibiting spermatogonia cell differentiation, we show that treatment with the differentiating factor all-trans retinoic acid induces a dramatic down-regulation of its expression. These results allow to conclude that, in the prepuberal testis, Nanos3 is important to maintain undifferentiated spermatogonia via the regulation of their cell cycle.

Links

PubMed Online version:10.1016/j.ydbio.2007.11.011

Keywords

Animals; Cell Line; Cells, Cultured; DNA, Complementary/genetics; Down-Regulation/drug effects; Embryo, Mammalian; Escherichia coli/genetics; Glutathione Transferase/metabolism; Green Fluorescent Proteins/metabolism; Humans; Immunomagnetic Separation; In Situ Hybridization; Kidney/cytology; Male; Mice; Mice, Inbred Strains; Octamer Transcription Factor-3/metabolism; Open Reading Frames; Plasmids; RNA, Messenger/metabolism; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; RNA-Binding Proteins/physiology; Recombinant Fusion Proteins/metabolism; Repressor Proteins/metabolism; Spermatogenesis; Spermatogonia/cytology; Spermatogonia/physiology; Testis/anatomy & histology; Testis/cytology; Testis/embryology; Testis/growth & development; Testis/metabolism; Time Factors; Transfection; Tretinoin/pharmacology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:NANO3

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q80U58

F

Seeded From UniProt

complete

MOUSE:PUM2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P60324

F

Seeded From UniProt

complete

MOUSE:NANO3

enables

GO:0003723: RNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:NANO3

involved_in

GO:0006417: regulation of translation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:NANO3

involved_in

GO:0007283: spermatogenesis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:NANO3

involved_in

GO:0051726: regulation of cell cycle

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.