PMID:18056639

Citation	Sarmah, B, Winfrey, VP, Olson, GE, Appel, B and Wente, SR (2007) A role for the inositol kinase Ipk1 in ciliary beating and length maintenance. Proc. Natl. Acad. Sci. U.S.A. 104:19843-8
Abstract	Cilia project from cells as membranous extensions, with microtubule structural cores assembling from basal bodies by intraflagellar transport (IFT). Here, we report a ciliary role for the inositol 1,3,4,5,6-pentakisphosphate 2-kinase (Ipk1) that generates inositol hexakisphosphate. In zebrafish embryos, reducing Ipk1 levels inhibited ciliary beating in Kupffer's vesicle and decreased ciliary length in the spinal canal, pronephric ducts, and Kupffer's vesicle. Electron microscopy showed that ciliary axonemal structures were not grossly altered. However, coincident knockdown of Ipk1 and IFT88 or IFT57 had synergistic perturbations. With GFP-Ipk1 enriched in centrosomes and basal bodies, we propose that Ipk1 plays a previously uncharacterized role in ciliary function.
Links	PubMed PMC2148385 Online version:10.1073/pnas.0706934104
Keywords	Animals; Animals, Genetically Modified; Biological Transport; Body Patterning; Cell Line; Centrosome/metabolism; Cilia/enzymology; Cilia/physiology; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Humans; Microtubules/metabolism; Phosphotransferases (Alcohol Group Acceptor)/genetics; Phosphotransferases (Alcohol Group Acceptor)/metabolism; Protozoan Proteins/genetics; Protozoan Proteins/metabolism; Zebrafish/embryology; Zebrafish/genetics; Zebrafish/metabolism; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism
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