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PMID:18020708

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Citation

Pesin, JA and Orr-Weaver, TL (2007) Developmental role and regulation of cortex, a meiosis-specific anaphase-promoting complex/cyclosome activator. PLoS Genet. 3:e202

Abstract

During oogenesis in metazoans, the meiotic divisions must be coordinated with development of the oocyte to ensure successful fertilization and subsequent embryogenesis. The ways in which the mitotic machinery is specialized for meiosis are not fully understood. cortex, which encodes a putative female meiosis-specific anaphase-promoting complex/cyclosome (APC/C) activator, is required for proper meiosis in Drosophila. We demonstrate that CORT physically associates with core subunits of the APC/C in ovaries. APC/C(CORT) targets Cyclin A for degradation prior to the metaphase I arrest, while Cyclins B and B3 are not targeted until after egg activation. We investigate the regulation of CORT and find that CORT protein is specifically expressed during the meiotic divisions in the oocyte. Polyadenylation of cort mRNA is correlated with appearance of CORT protein at oocyte maturation, while deadenylation of cort mRNA occurs in the early embryo. CORT protein is targeted for degradation by the APC/C following egg activation, and this degradation is dependent on an intact D-box in the C terminus of CORT. Our studies reveal the mechanism for developmental regulation of an APC/C activator and suggest it is one strategy for control of the female meiotic cell cycle in a multicellular organism.

Links

PubMed PMC2077894 Online version:10.1371/journal.pgen.0030202

Keywords

Amino Acid Sequence; Animals; Cell Cycle Proteins/chemistry; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cyclin A/metabolism; Drosophila Proteins/chemistry; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/cytology; Drosophila melanogaster/embryology; Drosophila melanogaster/genetics; Embryo, Nonmammalian/cytology; Embryo, Nonmammalian/metabolism; Embryonic Development; Female; Gene Expression Regulation, Developmental; Meiosis; Molecular Sequence Data; Oocytes/metabolism; Polyadenylation; Protein Processing, Post-Translational; RNA, Messenger/genetics; RNA, Messenger/metabolism; Substrate Specificity; Ubiquitin-Protein Ligase Complexes/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:CORT

enables

GO:0010997: anaphase-promoting complex binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0012058

F

Seeded From UniProt

complete

DROME:CORT

enables

GO:0010997: anaphase-promoting complex binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0025781

F

Seeded From UniProt

complete

DROME:CORT

involved_in

GO:1905786: positive regulation of anaphase-promoting complex-dependent catabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:CORT

involved_in

GO:1904668: positive regulation of ubiquitin protein ligase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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