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PMID:18003937

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Citation

Chekanova, JA, Abruzzi, KC, Rosbash, M and Belostotsky, DA (2008) Sus1, Sac3, and Thp1 mediate post-transcriptional tethering of active genes to the nuclear rim as well as to non-nascent mRNP. RNA 14:66-77

Abstract

Errors in the mRNP biogenesis pathway can lead to retention of mRNA in discrete, transcription-site-proximal foci. This RNA remains tethered adjacent to the transcription site long after transcriptional shutoff. Here we identify Sus1, Thp1, and Sac3 as factors required for the persistent tethering of such foci (dots) to their cognate genes. We also show that the prolonged association of previously activated GAL genes with the nuclear periphery after transcriptional shutoff is similarly dependent on the Sac3-Thp1-Sus1-Cdc31 complex. We suggest that the complex associates with nuclear mRNP and that mRNP properties influence the association of dot-confined mRNA with its gene of origin as well as the post-transcriptional retention of the cognate gene at the nuclear periphery. These findings indicate a coupling between the mRNA-to-gene and gene-to-nuclear periphery tethering. Taken together with other recent findings, these observations also highlight the importance of nuclear mRNP to the mobilization of active genes to the nuclear rim.

Links

PubMed PMC2151039 Online version:10.1261/rna.764108

Keywords

Cell Nucleus/genetics; In Situ Hybridization, Fluorescence; Nuclear Proteins/physiology; Nucleocytoplasmic Transport Proteins; Porins; RNA Processing, Post-Transcriptional; RNA-Binding Proteins/physiology; Ribonucleoproteins/genetics; Ribonucleoproteins/metabolism; Ribonucleoproteins/physiology; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:THP1

involved_in

GO:0000973: posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:THP1

involved_in

GO:0071033: nuclear retention of pre-mRNA at the site of transcription

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:SUS1

involved_in

GO:0000973: posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:SUS1

involved_in

GO:0071033: nuclear retention of pre-mRNA at the site of transcription

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:SAC3

involved_in

GO:0000973: posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:SAC3

involved_in

GO:0071033: nuclear retention of pre-mRNA at the site of transcription

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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