GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:17971504

From GONUTS
Jump to: navigation, search
Citation

McClintock, TS, Glasser, CE, Bose, SC and Bergman, DA (2008) Tissue expression patterns identify mouse cilia genes. Physiol. Genomics 32:198-206

Abstract

In mammals, cilia are critical for development, sensation, cell signaling, sperm motility, and fluid movement. Defects in cilia are causes of several congenital syndromes, providing additional reasons to identify cilia-related genes. We hypothesized that mRNAs selectively abundant in tissues rich in highly ciliated cells encode cilia proteins. Selective abundance in olfactory epithelium, testes, vomeronasal organ, trachea, and lung proved to be an expression pattern uniquely effective in identifying documented cilia-related genes. Known and suspected cilia-related genes were statistically overrepresented among the 99 genes identified, but the majority encoded proteins of unknown function, thereby predicting new cilia-related proteins. Evidence of expression in a highly ciliated cell, the olfactory sensory neuron, exists for 73 of the genes. In situ hybridization for 17 mRNAs confirmed expression of all 17 in olfactory sensory neurons. Most were also detected in vomeronasal sensory neurons and in neighboring tissues rich in ciliated cells such as respiratory epithelium. Immunoreactivity for one of the proteins identified, Spa17, colocalized with acetylated tubulin in the cilia layer of the olfactory epithelium. In contrast, the ciliary rootlet protein, Crocc, was located in discrete structures whose position was consistent with the dendritic knobs of the olfactory sensory neurons. A compilation of >2,000 mouse genes predicted to encode cilia-related proteins revealed a strong correlation (R = 0.99) between the number of studies predicting a gene's involvement in cilia and documented evidence of such involvement, a fact that simplifies the selection of genes for further study of the physiology of cilia.

Links

PubMed Online version:10.1152/physiolgenomics.00128.2007

Keywords

Animals; Cilia/genetics; Cilia/metabolism; Computational Biology; Female; Gene Expression Profiling; Immunohistochemistry; In Situ Hybridization; Male; Mice; Mice, Inbred C57BL; Neurons, Afferent/metabolism; Olfactory Mucosa/metabolism; Olfactory Receptor Neurons/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:SP17

located_in

GO:0005929: cilium

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000847)
  • part_of:(EMAPA:17606)|part_of:(CL:0002368)
  • part_of:(EMAPA:32827)

Seeded From UniProt

complete

MOUSE:PMP22

acts_upstream_of_or_within

GO:0031579: membrane raft organization

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3614822

P

Seeded From UniProt

complete

Notes

See also

References

See Help:References for how to manage references in GONUTS.