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PMID:17970746

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Citation

Sasai, N, Kato, Y, Kimura, G, Takeuchi, T and Yamaguchi, M (2007) The Drosophila jumonji gene encodes a JmjC-containing nuclear protein that is required for metamorphosis. FEBS J. 274:6139-51

Abstract

Jumonji (Jmj) is a transcriptional repressor that plays important roles in the suppression of cell proliferation and development of various tissues in the mouse. To further clarify the roles of Jmj during development and gain insight into mechanisms of Jmj-mediated transcriptional regulation, we have taken advantage of Drosophila as a model organism. Drosophila Jmj (dJmj) shares high homology with mammalian Jmj in the JmjN, JmjC and AT-rich interaction domains, as well as in the N-terminal repression domain. dJmj localizes to hundreds of euchromatic sites but not to chromocenter heterochromatin on salivary gland polytene chromosomes. In addition, dJmj is excluded from regions stained with an antibody against Ser5-phosphorylated RNA polymerase II, suggesting a function of dJmj in transcriptionally inactive chromatin. Loss of djmj results in larval and pupal lethality with phenotypes similar to those observed in mutants of ecdysone-regulated genes, implying the involvement of dJmj in the repression of gene expression in the ecdysone pathway. Transgenic mouse Jmj mostly colocalizes with dJmj and partially rescues the phenotypes of djmj mutants, indicating that dJmj is a functional homolog of mammalian Jmj. Furthermore, mutation in djmj suppresses position effect variegation of the T(2;3)Sb(V) rearrangement. These findings suggest that dJmj controls expression of developmentally important genes through modification of chromatin into a transcriptionally silenced state.

Links

PubMed Online version:10.1111/j.1742-4658.2007.06135.x

Keywords

Amino Acid Sequence; Animals; Animals, Genetically Modified; Cells, Cultured; Chromatin/genetics; Chromatin/metabolism; Drosophila/cytology; Drosophila/genetics; Drosophila/physiology; Drosophila Proteins/genetics; Drosophila Proteins/physiology; Euchromatin/metabolism; Gene Expression Regulation, Developmental; Gene Silencing; Genes, Insect; Immunohistochemistry; Metamorphosis, Biological/genetics; Metamorphosis, Biological/physiology; Molecular Sequence Data; Nuclear Proteins/chemistry; Nuclear Proteins/genetics; Nuclear Proteins/physiology; Protein Structure, Tertiary; Repressor Proteins; Salivary Glands/cytology; Salivary Glands/metabolism; Sequence Homology, Amino Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:Q6AWH4

located_in

GO:0000791: euchromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q8SXP6

located_in

GO:0000791: euchromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VT00

located_in

GO:0000791: euchromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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