PMID:17955443

Citation	Rashel, M, Uchiyama, J, Ujihara, T, Uehara, Y, Kuramoto, S, Sugihara, S, Yagyu, K, Muraoka, A, Sugai, M, Hiramatsu, K, Honke, K and Matsuzaki, S (2007) Efficient elimination of multidrug-resistant Staphylococcus aureus by cloned lysin derived from bacteriophage phi MR11. J. Infect. Dis. 196:1237-47
Abstract	We report the successful purification of a cloned lysin encoded by the novel Staphylococcus aureus bacteriophage phi MR11. The lysin, designated MV-L, rapidly and completely lysed cells of a number of S. aureus strains tested, including methicillin-resistant S. aureus (MRSA) and vancomycin-resistant S. aureus and a subset of vancomycin-intermediate S. aureus (VISA) in growing conditions. MV-L-mediated killing is specific to S. aureus and not to other species, except for S. simulans. MV-L exerted its staphylocidal effect synergistically with glycopeptide antibiotics against VISA. MV-L efficiently eliminated MRSA that had been artificially inoculated into the nares of mice. The intraperitoneal administration of MV-L also protected mice against MRSA septic death, without any harmful effects. Although MV-L evoked detectable levels of a humoral response in mice, the antibodies did not abolish the bacteriolytic activity. These results indicate that MV-L might be useful as a powerful therapeutic agent against multidrug-resistant S. aureus infections.
Links	PubMed Online version:10.1086/521305
Keywords	Administration, Intranasal; Administration, Topical; Animals; Anti-Bacterial Agents/administration & dosage; Anti-Bacterial Agents/isolation & purification; Bacterial Toxins/administration & dosage; Bacterial Toxins/isolation & purification; Disease Models, Animal; Drug Resistance, Multiple, Bacterial/drug effects; Endopeptidases/administration & dosage; Endopeptidases/biosynthesis; Female; Hemolysin Proteins/administration & dosage; Hemolysin Proteins/isolation & purification; Methicillin Resistance/drug effects; Mice; Recombinant Proteins/biosynthesis; Recombinant Proteins/isolation & purification; Staphylococcus Phages/metabolism; Staphylococcus aureus/drug effects; Staphylococcus aureus/virology; Vancomycin Resistance/drug effects
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BPMR1:Q08JW9		GO:0044659: cytolysis by virus of host cell	ECO:0000314:		P		Figure 2 (A-B) shows MV-L induced lysis of many bacterial strains compared to the buffer solution controls.	complete CACAO 10857
BPMR1:Q08JW9	involved_in	GO:0044659: cytolysis by virus of host cell	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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