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PMID:17951964

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Citation

Ichihara, K, Shimizu, H, Taguchi, O, Yamaguchi, M and Inoue, YH (2007) A Drosophila orthologue of larp protein family is required for multiple processes in male meiosis. Cell Struct. Funct. 32:89-100

Abstract

It is important for the proper execution of cell division in both mitosis and meiosis that the chromosome segregation, cytokinesis, and partition of cell organelles progress in smooth coordination. We show here that the mitochondria inheritance is closely linked with microtubules during meiotic divisions in Drosophila males. They are first clustered in a cell equator at metaphase associated with astral microtubules and then distributed along central spindle microtubules after anaphase. The molecular mechanism for the microtubule-dependent inheritance of mitochondria in male meiosis has not been demonstrated yet. We first isolated mutations for a larp gene that is highly conserved among eukaryotes and showed that these mutant males exhibited multiple meiotic phenotypes such as a failure of chromosome segregation, cytokinesis, and mitochondrial partition. Our cytological examination revealed that the mutants showed defects in spindle pole organization and spindle formation. The larp encodes a Drosophila orthologue of a La-related protein containing a domain exhibiting an outstanding homology with a La type RNA-binding protein. Surprisingly, the dLarp protein is localized in the cytoplasm of the male germ line cells, as observed by its distinct co-localization with mitochondria in early spermatocytes and during meiotic divisions. We discuss here the essential role that dLarp plays in multiple processes in Drosophila male meiosis.

Links

PubMed

Keywords

Animals; Centrosome/physiology; Chromatids/physiology; Chromosome Segregation; Cytokinesis; Drosophila/cytology; Drosophila/genetics; Drosophila/physiology; Drosophila Proteins/genetics; Drosophila Proteins/physiology; Male; Meiosis/genetics; Microtubules/physiology; Microtubules/ultrastructure; Mitochondria/genetics; Mitochondria/physiology; Mitotic Spindle Apparatus/genetics; Mitotic Spindle Apparatus/physiology; Mutant Proteins/physiology; Mutation; Spermatocytes/cytology; Spermatocytes/physiology; Transcription Factors/genetics; Transcription Factors/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:LARP

involved_in

GO:0000001: mitochondrion inheritance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:LARP

involved_in

GO:0007140: male meiotic nuclear division

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:LARP

involved_in

GO:0007053: spindle assembly involved in male meiosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:LARP

colocalizes_with

GO:0005739: mitochondrion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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