GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:17951129

From GONUTS
Jump to: navigation, search
Citation

Begon, E, Michel, L, Flageul, B, Beaudoin, I, Jean-Louis, F, Bachelez, H, Dubertret, L and Musette, P Expression, subcellular localization and cytokinic modulation of Toll-like receptors (TLRs) in normal human keratinocytes: TLR2 up-regulation in psoriatic skin. Eur J Dermatol 17:497-506

Abstract

The aim of the research described here was to investigate the expression of Toll-like receptors (TLRs) in normal human keratinocytes, to study its modulation by proinflammatory cytokines, and to characterize the function of the latter within the epidermis. Our results demonstrate that normal human keratinocytes may present an intra-cytoplasmic expression of TLR2, TLR3, and TLR4. Exposure of keratinocytes to IFN-gamma and TNF-alpha increased intra-cytoplasmic expression and led to partial translocation at the cell surface. Keratinocyte activation by TLR2, TLR3, and TLR4 ligands led to the nuclear translocation of NF-kappab and the release of proinflammatory cytokines TNF-alpha and IL-8. In immunochemistry analysis, psoriatic skin showed a strong over-expression of TLR2 in the epidermis compared with normal skin. Our results thus demonstrate large TLR expression in keratinocytes and the functionality of TLRs 2, 3, and 4. TLR2 over-expression in psoriatic skin provides new insights into TLR implication in the pathogenesis of psoriasis, through inappropriate stimulation by infectious or endogen ligands.

Links

PubMed Online version:10.1684/ejd.2007.0264

Keywords

Cells, Cultured; Electrophoretic Mobility Shift Assay; Humans; Immunologic Tests; Interferon-gamma/pharmacology; Interleukin-8/metabolism; Keratinocytes/cytology; Keratinocytes/drug effects; Keratinocytes/metabolism; Lipopolysaccharides/pharmacology; NF-kappa B/metabolism; Peptidoglycan/pharmacology; Poly I-C/pharmacology; Psoriasis/metabolism; RNA, Messenger/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Skin/drug effects; Skin/metabolism; Toll-Like Receptor 2/genetics; Toll-Like Receptor 2/metabolism; Toll-Like Receptors/genetics; Toll-Like Receptors/metabolism; Tumor Necrosis Factor-alpha/metabolism; Tumor Necrosis Factor-alpha/pharmacology; Up-Regulation

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:TLR4

involved_in

GO:0032760: positive regulation of tumor necrosis factor production

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:TLR4

involved_in

GO:0032757: positive regulation of interleukin-8 production

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:TLR4

involved_in

GO:0042535: positive regulation of tumor necrosis factor biosynthetic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:TLR4

involved_in

GO:0045416: positive regulation of interleukin-8 biosynthetic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

See Help:References for how to manage references in GONUTS.