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PMID:17921161

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Citation

Rylett, CM, Walker, MJ, Howell, GJ, Shirras, AD and Isaac, RE (2007) Male accessory glands of Drosophila melanogaster make a secreted angiotensin I-converting enzyme (ANCE), suggesting a role for the peptide-processing enzyme in seminal fluid. J. Exp. Biol. 210:3601-6

Abstract

Angiotensin I-converting enzyme (ACE) expressed on the surface of endothelial cells is responsible for the last step in the synthesis of circulating angiotensin II and the inactivation of bradykinin. Mammalian ACE is also expressed in the prostate with other components of the renin-angiotensin system, and in developing spermatids, where the peptidase activity is known to be critical for normal sperm function. The importance of an ACE gene to male fertility has also been demonstrated in Drosophila melanogaster, where Ance is expressed in spermatids, and hypomorphic alleles of Ance cause a defect in spermiogenesis. Here we show that ANCE, which shares many enzymatic properties with mammalian ACE, is also a product of the male accessory gland of D. melanogaster. It is expressed in the secondary cells and is associated with the electron dense granule within the large vesicles of these cells. ACE proteolytic activity is lost from the accessory glands during mating, consistent with transfer to the mated female in the seminal fluid. The accessory gland ACE-like activity might have an evolutionarily conserved function processing biologically active peptides with a role in male fertility.

Links

PubMed Online version:10.1242/jeb.009035

Keywords

Animal Structures/cytology; Animal Structures/enzymology; Animal Structures/ultrastructure; Animals; Drosophila melanogaster/cytology; Drosophila melanogaster/enzymology; Drosophila melanogaster/genetics; Drosophila melanogaster/ultrastructure; Gene Expression Regulation, Enzymologic; Immunohistochemistry; In Situ Hybridization; Male; Peptide Hydrolases/metabolism; Peptidyl-Dipeptidase A/genetics; Peptidyl-Dipeptidase A/secretion; Protein Processing, Post-Translational; Protein Transport; RNA, Messenger; Semen/enzymology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:ACE

involved_in

GO:0032504: multicellular organism reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:ACE

located_in

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:ACE

involved_in

GO:0006508: proteolysis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:ACE

enables

GO:0008241: peptidyl-dipeptidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:ACE

part_of

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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