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PMID:17917299

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Citation

Ohyama, K, Suzuki, M, Masuda, K, Yoshida, S and Muranaka, T (2007) Chemical phenotypes of the hmg1 and hmg2 mutants of Arabidopsis demonstrate the in-planta role of HMG-CoA reductase in triterpene biosynthesis. Chem. Pharm. Bull. 55:1518-21

Abstract

Plants produce a wide variety of cyclic triterpenes, such as sterols and triterpenoids, which are the major products of the mevalonate (MVA) pathway. It is important to understand the physiological functions of HMG-CoA reductase (HMGR) because HMGR is the rate-limiting enzyme in the MVA pathway. We have previously isolated Arabidopsis mutants in HMG1 and HMG2. Although the biochemical function of HMGR2 has been thought to be almost equal to that of HMGR1, based on similarities in their sequences, the phenotypes of mutants in these genes are quite different. Whereas hmg2 shows no abnormal phenotype under normal growth conditions, hmg1 shows pleiotropic phenotypes, including dwarfing, early senescence, and male sterility. We previously postulated that the 50% decrease in the sterol content of hmg1, as compared to that in the wild type, was a cause of these phenotypes, but comprehensive triterpene profiles of these mutants had not yet been determined. Here, we present the triterpene profiles of hmg1 and hmg2. In contrast to hmg1, hmg2 showed a sterol content 15% lower than that of the wild type. A precise triterpenoid quantification using synthesized deuterated compounds of beta-amyrin (1), alpha-amyrin (2), and lupeol (3) showed that the levels of triterpenoids in hmg1 and hmg2 were 65% and 25% lower than in the wild type (WT), respectively. These results demonstrate that HMGR2 as well as HMGR1 is responsible for the biosynthesis of triterpenes in spite of the lack of visible phenotypes in hmg2.

Links

PubMed

Keywords

Arabidopsis/enzymology; Arabidopsis/genetics; Chromatography, Gas; Deuterium/chemistry; Genes, Plant; HMGB1 Protein/genetics; HMGB1 Protein/metabolism; HMGB2 Protein/genetics; HMGB2 Protein/metabolism; Hydroxymethylglutaryl CoA Reductases/genetics; Hydroxymethylglutaryl CoA Reductases/metabolism; Mevalonic Acid/metabolism; Mutation; Oleanolic Acid/analogs & derivatives; Oleanolic Acid/chemical synthesis; Oleanolic Acid/metabolism; Pentacyclic Triterpenes; Phenotype; Sterols/metabolism; Triterpenes/chemical synthesis; Triterpenes/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:HMDH2

acts_upstream_of_or_within

GO:0016104: triterpenoid biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:HMDH2

acts_upstream_of_or_within

GO:0016126: sterol biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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