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PMID:17908918

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Citation

Couwenbergs, C, Labbé, JC, Goulding, M, Marty, T, Bowerman, B and Gotta, M (2007) Heterotrimeric G protein signaling functions with dynein to promote spindle positioning in C. elegans. J. Cell Biol. 179:15-22

Abstract

Proper orientation and positioning of the mitotic spindle is essential for the correct segregation of fate determinants during asymmetric cell division. Although heterotrimeric G proteins and their regulators are essential for spindle positioning in many cell types, their mechanism of action remains unclear. In this study, we show that dyrb-1, which encodes a dynein light chain, provides a functional link between heterotrimeric G protein signaling and dynein activity during spindle positioning in Caenorhabditis elegans. Embryos depleted of dyrb-1 display phenotypes similar to a weak loss of function of dynein activity, indicating that DYRB-1 is a positive regulator of dynein. We find that the depletion of dyrb-1 enhances the spindle positioning defect of weak loss of function alleles of two regulators of G protein signaling, LIN-5 and GPR-1/2, and that DYRB-1 physically associates with these two proteins. These results indicate that dynein activity functions with regulators of G protein signaling to regulate common downstream effectors during spindle positioning in the early C. elegans embryo.

Links

PubMed PMC2064726 Online version:10.1083/jcb.200707085

Keywords

Animals; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caenorhabditis elegans/ultrastructure; Caenorhabditis elegans Proteins/antagonists & inhibitors; Caenorhabditis elegans Proteins/metabolism; Caenorhabditis elegans Proteins/physiology; Dyneins/antagonists & inhibitors; Dyneins/metabolism; Dyneins/physiology; Embryo, Nonmammalian/metabolism; Embryo, Nonmammalian/ultrastructure; Green Fluorescent Proteins/analysis; Heterotrimeric GTP-Binding Proteins/metabolism; Heterotrimeric GTP-Binding Proteins/physiology; Mitotic Spindle Apparatus/metabolism; Mitotic Spindle Apparatus/ultrastructure; Models, Biological; RNA Interference; Recombinant Fusion Proteins/analysis; Signal Transduction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:Q22752

located_in

GO:0005813: centrosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:Q22752

located_in

GO:0048471: perinuclear region of cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:Q22752

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:Q22752

located_in

GO:0005876: spindle microtubule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:Q22752

located_in

GO:0005938: cell cortex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:Q22752

located_in

GO:0005694: chromosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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