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PMID:17888405

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Citation

Kawai, M, Mushiake, S, Bessho, K, Murakami, M, Namba, N, Kokubu, C, Michigami, T and Ozono, K (2007) Wnt/Lrp/beta-catenin signaling suppresses adipogenesis by inhibiting mutual activation of PPARgamma and C/EBPalpha. Biochem. Biophys. Res. Commun. 363:276-82

Abstract

Wnt/beta-catenin signaling has been implicated in repressing adipogenesis. Several lines of evidence show that the possible mechanism is blockade of PPARgamma induction. However, the precise mechanisms remain to be elucidated. In this study, we demonstrated that Wnt3a conditioned medium suppresses C/EBPbeta/delta-induced adipogenesis of 3T3-L1 cells by inhibiting PPARgamma induction. In addition, the mutual activation of PPARgamma and C/EBPalpha was also repressed in the presence of Wnt3a. To further investigate the role of the canonical Wnt pathway in adipogenesis, we used mouse embryonic fibroblasts (MEFs) isolated from Lrp6-deficient embryos. Contrary to wild-type MEFs, Lrp6-deficient MEFs showed spontaneous adipogenesis and escaped the suppressive effect of exogenous Wnt3a. These findings suggest a critical role of Wnt/Lrp6/beta-catenin signaling in adipogenesis and cell fate decision of mesenchymal stem cells.

Links

PubMed Online version:10.1016/j.bbrc.2007.08.088

Keywords

3T3-L1 Cells; Adipocytes/metabolism; Adipogenesis/physiology; Animals; CCAAT-Enhancer-Binding Protein-beta/metabolism; LDL-Receptor Related Proteins/metabolism; Low Density Lipoprotein Receptor-Related Protein-6; Mice; PPAR gamma/metabolism; Signal Transduction/physiology; Wnt Proteins/metabolism; beta Catenin/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:LRP6

involved_in

GO:0045444: fat cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2156191

P

Seeded From UniProt

complete

MOUSE:LRP6

involved_in

GO:0045599: negative regulation of fat cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2156191

P

Seeded From UniProt

complete

MOUSE:WNT3A

involved_in

GO:0045444: fat cell differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:WNT3A

involved_in

GO:0045599: negative regulation of fat cell differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:WNT3A

involved_in

GO:0060070: canonical Wnt signaling pathway

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:WNT3A

involved_in

GO:0045444: fat cell differentiation

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:103573

P

Seeded From UniProt

complete

MOUSE:WNT3A

involved_in

GO:0045444: fat cell differentiation

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:88373

P

Seeded From UniProt

complete

MOUSE:WNT3A

involved_in

GO:0045599: negative regulation of fat cell differentiation

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:103573

P

Seeded From UniProt

complete

MOUSE:WNT3A

involved_in

GO:0045599: negative regulation of fat cell differentiation

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:88373

P

Seeded From UniProt

complete

MOUSE:CEBPB

involved_in

GO:0045444: fat cell differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CEBPB

involved_in

GO:0045444: fat cell differentiation

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:98956

P

Seeded From UniProt

complete

MOUSE:CEBPD

involved_in

GO:0045444: fat cell differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CEBPD

involved_in

GO:0045444: fat cell differentiation

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:98956

P

Seeded From UniProt

complete

MOUSE:WNT3A

acts_upstream_of_or_within

GO:0045599: negative regulation of fat cell differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

regulates_o_results_in_acquisition_of_features_of:(CL:0000136)

Seeded From UniProt

complete

MOUSE:WNT3A

acts_upstream_of_or_within

GO:0045599: negative regulation of fat cell differentiation

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:103573
MGI:MGI:88373

P

regulates_o_results_in_acquisition_of_features_of:(CL:0000136)

Seeded From UniProt

complete

MOUSE:WNT3A

involved_in

GO:0060070: canonical Wnt signaling pathway

ECO:0000314: direct assay evidence used in manual assertion

P

occurs_in:(CL:0002334)

Seeded From UniProt

complete


See also

References

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