PMID:17888405

Citation	Kawai, M, Mushiake, S, Bessho, K, Murakami, M, Namba, N, Kokubu, C, Michigami, T and Ozono, K (2007) Wnt/Lrp/beta-catenin signaling suppresses adipogenesis by inhibiting mutual activation of PPARgamma and C/EBPalpha. Biochem. Biophys. Res. Commun. 363:276-82
Abstract	Wnt/beta-catenin signaling has been implicated in repressing adipogenesis. Several lines of evidence show that the possible mechanism is blockade of PPARgamma induction. However, the precise mechanisms remain to be elucidated. In this study, we demonstrated that Wnt3a conditioned medium suppresses C/EBPbeta/delta-induced adipogenesis of 3T3-L1 cells by inhibiting PPARgamma induction. In addition, the mutual activation of PPARgamma and C/EBPalpha was also repressed in the presence of Wnt3a. To further investigate the role of the canonical Wnt pathway in adipogenesis, we used mouse embryonic fibroblasts (MEFs) isolated from Lrp6-deficient embryos. Contrary to wild-type MEFs, Lrp6-deficient MEFs showed spontaneous adipogenesis and escaped the suppressive effect of exogenous Wnt3a. These findings suggest a critical role of Wnt/Lrp6/beta-catenin signaling in adipogenesis and cell fate decision of mesenchymal stem cells.
Links	PubMed Online version:10.1016/j.bbrc.2007.08.088
Keywords	3T3-L1 Cells; Adipocytes/metabolism; Adipogenesis/physiology; Animals; CCAAT-Enhancer-Binding Protein-beta/metabolism; LDL-Receptor Related Proteins/metabolism; Low Density Lipoprotein Receptor-Related Protein-6; Mice; PPAR gamma/metabolism; Signal Transduction/physiology; Wnt Proteins/metabolism; beta Catenin/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:LRP6	involved_in	GO:0045444: fat cell differentiation	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2156191	P		Seeded From UniProt	complete
MOUSE:LRP6	involved_in	GO:0045599: negative regulation of fat cell differentiation	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2156191	P		Seeded From UniProt	complete
MOUSE:WNT3A	involved_in	GO:0045444: fat cell differentiation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:WNT3A	involved_in	GO:0045599: negative regulation of fat cell differentiation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:WNT3A	involved_in	GO:0060070: canonical Wnt signaling pathway	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:WNT3A	involved_in	GO:0045444: fat cell differentiation	ECO:0000316: genetic interaction evidence used in manual assertion	MGI:MGI:103573	P		Seeded From UniProt	complete
MOUSE:WNT3A	involved_in	GO:0045444: fat cell differentiation	ECO:0000316: genetic interaction evidence used in manual assertion	MGI:MGI:88373	P		Seeded From UniProt	complete
MOUSE:WNT3A	involved_in	GO:0045599: negative regulation of fat cell differentiation	ECO:0000316: genetic interaction evidence used in manual assertion	MGI:MGI:103573	P		Seeded From UniProt	complete
MOUSE:WNT3A	involved_in	GO:0045599: negative regulation of fat cell differentiation	ECO:0000316: genetic interaction evidence used in manual assertion	MGI:MGI:88373	P		Seeded From UniProt	complete
MOUSE:CEBPB	involved_in	GO:0045444: fat cell differentiation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:CEBPB	involved_in	GO:0045444: fat cell differentiation	ECO:0000316: genetic interaction evidence used in manual assertion	MGI:MGI:98956	P		Seeded From UniProt	complete
MOUSE:CEBPD	involved_in	GO:0045444: fat cell differentiation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:CEBPD	involved_in	GO:0045444: fat cell differentiation	ECO:0000316: genetic interaction evidence used in manual assertion	MGI:MGI:98956	P		Seeded From UniProt	complete
MOUSE:WNT3A	acts_upstream_of_or_within	GO:0045599: negative regulation of fat cell differentiation	ECO:0000314: direct assay evidence used in manual assertion		P	regulates_o_results_in_acquisition_of_features_of:(CL:0000136)	Seeded From UniProt	complete
MOUSE:WNT3A	acts_upstream_of_or_within	GO:0045599: negative regulation of fat cell differentiation	ECO:0000316: genetic interaction evidence used in manual assertion	MGI:MGI:103573 MGI:MGI:88373	P	regulates_o_results_in_acquisition_of_features_of:(CL:0000136)	Seeded From UniProt	complete
MOUSE:WNT3A	involved_in	GO:0060070: canonical Wnt signaling pathway	ECO:0000314: direct assay evidence used in manual assertion		P	occurs_in:(CL:0002334)	Seeded From UniProt	complete

References

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