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PMID:17875665

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Citation

Brower-Toland, B, Findley, SD, Jiang, L, Liu, L, Yin, H, Dus, M, Zhou, P, Elgin, SC and Lin, H (2007) Drosophila PIWI associates with chromatin and interacts directly with HP1a. Genes Dev. 21:2300-11

Abstract

The interface between cellular systems involving small noncoding RNAs and epigenetic change remains largely unexplored in metazoans. RNA-induced silencing systems have the potential to target particular regions of the genome for epigenetic change by locating specific sequences and recruiting chromatin modifiers. Noting that several genes encoding RNA silencing components have been implicated in epigenetic regulation in Drosophila, we sought a direct link between the RNA silencing system and heterochromatin components. Here we show that PIWI, an ARGONAUTE/PIWI protein family member that binds to Piwi-interacting RNAs (piRNAs), strongly and specifically interacts with heterochromatin protein 1a (HP1a), a central player in heterochromatic gene silencing. The HP1a dimer binds a PxVxL-type motif in the N-terminal domain of PIWI. This motif is required in fruit flies for normal silencing of transgenes embedded in heterochromatin. We also demonstrate that PIWI, like HP1a, is itself a chromatin-associated protein whose distribution in polytene chromosomes overlaps with HP1a and appears to be RNA dependent. These findings implicate a direct interaction between the PIWI-mediated small RNA mechanism and heterochromatin-forming pathways in determining the epigenetic state of the fly genome.

Links

PubMed PMC1973144 Online version:10.1101/gad.1564307

Keywords

Amino Acid Motifs; Animals; Argonaute Proteins; Binding Sites; Chromatin/metabolism; Chromosomal Proteins, Non-Histone/metabolism; Drosophila Proteins/metabolism; Drosophila melanogaster/genetics; Embryo, Nonmammalian; Female; Gene Silencing; Male; Models, Molecular; Nuclear Proteins/metabolism; Protein Binding; Proteins/chemistry; Proteins/metabolism; Proteins/physiology; RNA-Induced Silencing Complex

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:PIWI

located_in

GO:0010369: chromocenter

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:PIWI

involved_in

GO:0006342: chromatin silencing

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:PIWI

located_in

GO:0000791: euchromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:PIWI

located_in

GO:0000792: heterochromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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