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PMID:17869112

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Citation

Maruyama, R, Velarde, NV, Klancer, R, Gordon, S, Kadandale, P, Parry, JM, Hang, JS, Rubin, J, Stewart-Michaelis, A, Schweinsberg, P, Grant, BD, Piano, F, Sugimoto, A and Singson, A (2007) EGG-3 regulates cell-surface and cortex rearrangements during egg activation in Caenorhabditis elegans. Curr. Biol. 17:1555-60

Abstract

Fertilization triggers egg activation and converts the egg into a developing embryo. The events of this egg-to-embryo transition typically include the resumption of meiosis, the reorganization of the cortical actin cytoskeleton, and the remodeling of the oocyte surface. The factors that regulate sperm-dependent egg-activation events are not well understood. Caenorhabditis elegans EGG-3, a member of the protein tyrosine phosphatase-like (PTPL) family, is essential for regulating cell-surface and cortex rearrangements during egg activation in response to sperm entry. Although fertilization occurred normally in egg-3 mutants, the polarized dispersal of F-actin is altered, a chitin eggshell is not formed, and no polar bodies are produced. EGG-3 is associated with the oocyte plasma membrane in a pattern that is similar to CHS-1 and MBK-2. CHS-1 is required for eggshell deposition, whereas MBK-2 is required for the degradation of maternal proteins during the egg-to-embryo transition. The localization of CHS-1 and EGG-3 are interdependent and both genes were required for the proper localization of MBK-2 in oocytes. Therefore, EGG-3 plays a central role in egg activation by influencing polarized F-actin dynamics and the localization or activity of molecules that are directly involved in executing the egg-to-embryo transition.

Links

PubMed Online version:10.1016/j.cub.2007.08.011

Keywords

Actins/analysis; Actins/metabolism; Amino Acid Motifs; Animals; Caenorhabditis elegans/embryology; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/analysis; Caenorhabditis elegans Proteins/chemistry; Caenorhabditis elegans Proteins/metabolism; Caenorhabditis elegans Proteins/physiology; Cell Membrane/metabolism; Embryo, Nonmammalian/cytology; Embryo, Nonmammalian/metabolism; Fertilization; Green Fluorescent Proteins/analysis; Molecular Sequence Data; Ovum/cytology; Ovum/growth & development; Ovum/metabolism; Protein Tyrosine Phosphatases/chemistry; Protein Tyrosine Phosphatases/metabolism; Protein Tyrosine Phosphatases/physiology; Protein-Tyrosine Kinases/analysis; Protein-Tyrosine Kinases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:CHS1

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:CHS1

involved_in

GO:1904778: positive regulation of protein localization to cell cortex

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • has_input:(UniProtKB:Q20402)
  • occurs_in:(WBbt:0006797)

Seeded From UniProt

complete

CAEEL:EGG3

located_in

GO:0005938: cell cortex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:EGG3

involved_in

GO:0030866: cortical actin cytoskeleton organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:EGG3

involved_in

GO:1904778: positive regulation of protein localization to cell cortex

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • has_input:(UniProtKB:G5ECD6)
  • occurs_in:(WBbt:0006797)|has_input(UniProtKB:Q9XTF3)
  • occurs_in:(WBbt:0006797)

Seeded From UniProt

complete

CAEEL:EGG3

involved_in

GO:0040038: polar body extrusion after meiotic divisions

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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