GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:17855365

From GONUTS
Jump to: navigation, search
Citation

Li, YF, He, W, Jha, KN, Klotz, K, Kim, YH, Mandal, A, Pulido, S, Digilio, L, Flickinger, CJ and Herr, JC (2007) FSCB, a novel protein kinase A-phosphorylated calcium-binding protein, is a CABYR-binding partner involved in late steps of fibrous sheath biogenesis. J. Biol. Chem. 282:34104-19

Abstract

We report characterization of a novel testis- and sperm-specific protein, FSCB (fibrous sheath CABYR binding), that is expressed post-meiotically and localized in mouse sperm flagella. FSCB was identified as a binding partner of CABYR, a calcium-binding protein that is tyrosine-phosphorylated during capacitation. Orthologous genes of FSCB are present in other mammals, including rat and human, and conserved motifs in FSCB include PXXP, proline-rich and extensin-like regions. FSCB is phosphorylated by protein kinase A as shown by in vitro phosphorylation assay and also by determining phosphorylation sites in native FSCB from mouse sperm. Calcium overlay assay showed that FSCB is a calcium-binding protein from sperm. FSCB is a post meiotic protein first expressed at step 11 of mouse spermatogenesis in the elongating spermatids, and it subsequently incorporates into the flagellar principal piece of the sperm. Ultrastructurally, FSCB localized to a cortical layer of intermediate electron density at the surface of the ribs and longitudinal columns of the fibrous sheath. Due to its temporal appearance during spermiogenesis and location at the cortex of the fibrous sheath, FSCB is postulated to be involved in the later stages of fibrous sheath assembly.

Links

PubMed Online version:10.1074/jbc.M702238200

Keywords

Amino Acid Motifs/genetics; Animals; Base Sequence; Calcium/metabolism; Calcium-Binding Proteins/biosynthesis; Calcium-Binding Proteins/genetics; Calcium-Binding Proteins/metabolism; Cyclic AMP-Dependent Protein Kinases/metabolism; Humans; Male; Mice; Molecular Sequence Data; Phosphoproteins/genetics; Phosphoproteins/metabolism; Phosphorylation; Rats; Sperm Capacitation/physiology; Sperm Tail/metabolism; Sperm Tail/ultrastructure; Spermatids/metabolism; Spermatids/ultrastructure; Spermatogenesis/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:FSCB

enables

GO:0005509: calcium ion binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:FSCB

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q9D424

F

  • occurs_in:(EMAPA:17972)

Seeded From UniProt

complete

MOUSE:FSCB

located_in

GO:0097228: sperm principal piece

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000019)

Seeded From UniProt

complete

MOUSE:FSCB

located_in

GO:0035686: sperm fibrous sheath

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000019)

Seeded From UniProt

complete

MOUSE:KAPCA

acts_upstream_of_or_within

GO:0006468: protein phosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:KAPCA

enables

GO:0004672: protein kinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_input:(MGI:MGI:3646964)

Seeded From UniProt

complete

MOUSE:CABYR

enables

GO:0005509: calcium ion binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:CABYR

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:A1EGX6

F

  • occurs_in:(EMAPA:17972)

Seeded From UniProt

complete

MOUSE:CABYR

located_in

GO:0097228: sperm principal piece

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000019)

Seeded From UniProt

complete

MOUSE:CABYR

located_in

GO:0097229: sperm end piece

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:KAPCA

acts_upstream_of_or_within

GO:0046777: protein autophosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:KAPCA

acts_upstream_of_or_within

GO:0006468: protein phosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

has_participant:(MGI:MGI:3646964)

Seeded From UniProt

complete

MOUSE:KAPCA

enables

GO:0004672: protein kinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

has_direct_input:(MGI:MGI:3646964)

Seeded From UniProt

complete

See also

References

See Help:References for how to manage references in GONUTS.