GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:17848950

From GONUTS
Jump to: navigation, search
Citation

Cheng, YY, Yu, J, Wong, YP, Man, EP, To, KF, Jin, VX, Li, J, Tao, Q, Sung, JJ, Chan, FK and Leung, WK (2007) Frequent epigenetic inactivation of secreted frizzled-related protein 2 (SFRP2) by promoter methylation in human gastric cancer. Br. J. Cancer 97:895-901

Abstract

The role of secreted frizzled-related protein (SFRP) genes in gastric cancer remains largely unknown. We determined the frequency and functional significance of SFRPs hypermethylation in human gastric cancer. The expression and methylation status of four SFRP members (SFRP1, 2, 4, and 5) in primary gastric cancer samples was screened. The biological effects of SFRP were analysed by flow cytometry, cell viability assay and in vivo tumour growth in nude mice. Among the four SFRPs, only SFRP2 was significantly downregulated in gastric cancer as compared to adjacent non-cancer samples (P<0.01). Promoter hypermethylation of SFRP2 was detected in 73.3% primary gastric cancer tissues, 37.5% of samples showing intestinal metaplasia and 20% adjacent normal gastric tissues. Bisulphite DNA sequencing confirmed the densely methylated SFRP2 promoter region. Demethylation treatment restored the expression of SFRP2 in gastric cancer cell lines. Forced expression of SFRP2 induced cell apoptosis, inhibited proliferation of gastric cancer cells and suppressed tumour growth in vivo. Moreover, methylated SFRP2 was detected in 66.7% of serum samples from cancer patients but not in normal controls. In conclusion, epigenetic inactivation of SFRP2 is a common and early event contributing to gastric carcinogenesis and may be a potential biomarker for gastric cancer.

Links

PubMed PMC2360406 Online version:10.1038/sj.bjc.6603968

Keywords

Adenocarcinoma/genetics; Adenocarcinoma/metabolism; Animals; Apoptosis; Azacitidine; Cell Line, Tumor; Cell Proliferation; DNA Methylation; DNA Primers/chemistry; Epigenesis, Genetic; Eye Proteins/genetics; Eye Proteins/metabolism; Flow Cytometry; Gene Expression Regulation, Neoplastic; Gene Silencing; Humans; Intercellular Signaling Peptides and Proteins/genetics; Intercellular Signaling Peptides and Proteins/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mice; Mice, Nude; Promoter Regions, Genetic; Proto-Oncogene Proteins/genetics; Proto-Oncogene Proteins/metabolism; Stomach Neoplasms/genetics; Stomach Neoplasms/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:SFRP2

involved_in

GO:0008285: negative regulation of cell population proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:SFRP2

involved_in

GO:0043065: positive regulation of apoptotic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.