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PMID:17827154

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Citation

Sun, JM, Chen, HY and Davie, JR (2007) Differential distribution of unmodified and phosphorylated histone deacetylase 2 in chromatin. J. Biol. Chem. 282:33227-36

Abstract

Histone deacetylase 2 (HDAC2) is one of the histone-modifying enzymes that regulate gene expression by remodeling chromatin structure. Along with HDAC1, HDAC2 is found in the Sin3 and NuRD multiprotein complexes, which are recruited to promoters by DNA-binding proteins. In this study, we show that the majority of HDAC2 in human breast cancer cells is not phosphorylated. However, the minor population of HDAC2, preferentially cross-linked to DNA by cisplatin, is mono-, di-, or tri-phosphorylated. Furthermore, HDAC2 phosphorylation is required for formation of Sin3 and NuRD complexes and recruitment to promoters by transcription factors including p53, Rb, YY1, NF-kappaB, Sp1, and Sp3. Unmodified HDAC2 requires linker DNA to associate with chromatin but is not cross-linked to DNA by formaldehyde. We provide evidence that unmodified HDAC2 is associated with the coding region of transcribed genes, whereas phosphorylated HDAC2 is primarily recruited to promoters.

Links

PubMed Online version:10.1074/jbc.M703549200

Keywords

Amino Acid Sequence; Animals; Carrier Proteins/metabolism; Cell Line; Chickens; Chromatin/enzymology; Chromatin/metabolism; Cross-Linking Reagents/chemistry; DNA/chemistry; DNA/genetics; DNA-Binding Proteins/metabolism; Formaldehyde; Globins/genetics; Globins/metabolism; Histone Deacetylase 2; Histone Deacetylases/chemistry; Histone Deacetylases/genetics; Histone Deacetylases/metabolism; Humans; Molecular Sequence Data; Nuclear Proteins/metabolism; Phosphorylation; Promoter Regions, Genetic/genetics; Repressor Proteins/chemistry; Repressor Proteins/genetics; Repressor Proteins/metabolism; Retinoblastoma-Binding Protein 4; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:MBD3

part_of

GO:0016581: NuRD complex

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

HUMAN:SP1

enables

GO:0042826: histone deacetylase binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q92769

F

Seeded From UniProt

complete

HUMAN:CHD3

part_of

GO:0016581: NuRD complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:HDAC2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q09028

F

Seeded From UniProt

complete

HUMAN:HDAC2

enables

GO:0061629: RNA polymerase II-specific DNA-binding transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P08047

F

Seeded From UniProt

complete

HUMAN:HDAC2

enables

GO:0061629: RNA polymerase II-specific DNA-binding transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q02086

F

Seeded From UniProt

complete

HUMAN:SIN3A

part_of

GO:0016580: Sin3 complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:SP2

enables

GO:0042826: histone deacetylase binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q92769

F

Seeded From UniProt

complete

HUMAN:CSK21

part_of

GO:0016581: NuRD complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CSK21

part_of

GO:0016580: Sin3 complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:HDAC2

part_of

GO:0016581: NuRD complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:HDAC2

part_of

GO:0016580: Sin3 complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:HDAC2

enables

GO:0008134: transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q02086

F

Seeded From UniProt

complete

HUMAN:HDAC2

enables

GO:0008134: transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P08047

F

Seeded From UniProt

complete

HUMAN:HDAC2

involved_in

GO:0006338: chromatin remodeling

ECO:0000305: curator inference used in manual assertion

GO:0004407

P

Seeded From UniProt

complete

HUMAN:HDAC1

part_of

GO:0016581: NuRD complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:HDAC1

part_of

GO:0016580: Sin3 complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:HDAC1

involved_in

GO:0006338: chromatin remodeling

ECO:0000305: curator inference used in manual assertion

GO:0004407

P

Seeded From UniProt

complete

HUMAN:RBBP4

enables

GO:0042826: histone deacetylase binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q92769

F

Seeded From UniProt

complete

HUMAN:RBBP4

enables

GO:0042393: histone binding

ECO:0000303: author statement without traceable support used in manual assertion

F

Seeded From UniProt

complete

HUMAN:RBBP4

part_of

GO:0016581: NuRD complex

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

HUMAN:RBBP4

part_of

GO:0016580: Sin3 complex

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete


See also

References

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