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PMID:17805344

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Citation

Grainge, I, Bregu, M, Vazquez, M, Sivanathan, V, Ip, SC and Sherratt, DJ (2007) Unlinking chromosome catenanes in vivo by site-specific recombination. EMBO J. 26:4228-38

Abstract

A challenge for chromosome segregation in all domains of life is the formation of catenated progeny chromosomes, which arise during replication as a consequence of the interwound strands of the DNA double helix. Topoisomerases play a key role in DNA unlinking both during and at the completion of replication. Here we report that chromosome unlinking can instead be accomplished by multiple rounds of site-specific recombination. We show that step-wise, site-specific recombination by XerCD-dif or Cre-loxP can unlink bacterial chromosomes in vivo, in reactions that require KOPS-guided DNA translocation by FtsK. Furthermore, we show that overexpression of a cytoplasmic FtsK derivative is sufficient to allow chromosome unlinking by XerCD-dif recombination when either subunit of TopoIV is inactivated. We conclude that FtsK acts in vivo to simplify chromosomal topology as Xer recombination interconverts monomeric and dimeric chromosomes.

Links

PubMed PMC2230843 Online version:10.1038/sj.emboj.7601849

Keywords

Chromosomes, Bacterial/genetics; Chromosomes, Bacterial/metabolism; DNA Replication/physiology; DNA Topoisomerase IV/genetics; DNA Topoisomerase IV/metabolism; DNA, Catenated/genetics; DNA, Catenated/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Integrases/genetics; Integrases/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Recombination, Genetic/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:FTSK

involved_in

GO:0007059: chromosome segregation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

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