GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:17803941

From GONUTS
Jump to: navigation, search
Citation

Bradatsch, B, Katahira, J, Kowalinski, E, Bange, G, Yao, W, Sekimoto, T, Baumgärtel, V, Boese, G, Bassler, J, Wild, K, Peters, R, Yoneda, Y, Sinning, I and Hurt, E (2007) Arx1 functions as an unorthodox nuclear export receptor for the 60S preribosomal subunit. Mol. Cell 27:767-79

Abstract

Shuttling transport receptors carry cargo through nuclear pore complexes (NPCs) via transient interactions with Phe-Gly (FG)-rich nucleoporins. Here, we identify Arx1, a factor associated with a late 60S preribosomal particle in the nucleus, as an unconventional export receptor. Arx1 binds directly to FG nucleoporins and exhibits facilitated translocation through NPCs. Moreover, Arx1 functionally overlaps with the other 60S export receptors, Xpo1 and Mex67-Mtr2, and is genetically linked to nucleoporins. Unexpectedly, Arx1 is structurally unrelated to known shuttling transport receptors but homologous to methionine aminopeptidases (MetAPs), however, without enzymatic activity. Typically, the MetAP fold creates a central cavity that binds the methionine. In contrast, the predicted central cavity of Arx1 is involved in the interaction with FG repeat nucleoporins and 60S subunit export. Thus, an ancient enzyme fold has been adopted by Arx1 to function as a nuclear export receptor.

Links

PubMed Online version:10.1016/j.molcel.2007.06.034

Keywords

Active Transport, Cell Nucleus/physiology; Amino Acid Sequence; Cell Nucleus/metabolism; Fungal Proteins/chemistry; Fungal Proteins/physiology; HeLa Cells; Humans; Molecular Sequence Data; Nuclear Pore/metabolism; Nuclear Pore Complex Proteins/chemistry; Nuclear Pore Complex Proteins/metabolism; Nuclear Pore Complex Proteins/physiology; Protein Structure, Tertiary; Receptors, Cytoplasmic and Nuclear/chemistry; Receptors, Cytoplasmic and Nuclear/physiology; Ribosomal Proteins/metabolism; Ribosomes/metabolism; Sequence Alignment

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:NUP42

enables

GO:0017056: structural constituent of nuclear pore

ECO:0000353: physical interaction evidence used in manual assertion

SGD:S000002508

F

Seeded From UniProt

complete

YEAST:NU100

enables

GO:0017056: structural constituent of nuclear pore

ECO:0000353: physical interaction evidence used in manual assertion

SGD:S000002508

F

Seeded From UniProt

complete

YEAST:NU116

enables

GO:0017056: structural constituent of nuclear pore

ECO:0000353: physical interaction evidence used in manual assertion

SGD:S000002508

F

Seeded From UniProt

complete

YEAST:ARX1

involved_in

GO:0000055: ribosomal large subunit export from nucleus

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000055
SGD:S000000909
SGD:S000001213
SGD:S000001669
SGD:S000003450
SGD:S000006090

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.