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PMID:17785412

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Citation

Brosnan, CA, Mitter, N, Christie, M, Smith, NA, Waterhouse, PM and Carroll, BJ (2007) Nuclear gene silencing directs reception of long-distance mRNA silencing in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. 104:14741-6

Abstract

In plants, silencing of mRNA can be transmitted from cell to cell and also over longer distances from roots to shoots. To investigate the long-distance mechanism, WT and mutant shoots were grafted onto roots silenced for an mRNA. We show that three genes involved in a chromatin silencing pathway, NRPD1a encoding RNA polymerase IVa, RNA-dependent RNA polymerase 2 (RDR2), and DICER-like 3 (DCL3), are required for reception of long-distance mRNA silencing in the shoot. A mutant representing a fourth gene in the pathway, argonaute4 (ago4), was also partially compromised in the reception of silencing. This pathway produces 24-nt siRNAs and resulted in decapped RNA, a known substrate for amplification of dsRNA by RDR6. Activation of silencing in grafted shoots depended on RDR6, but no 24-nt siRNAs were detected in mutant rdr6 shoots, indicating that RDR6 also plays a role in initial signal perception. After amplification of decapped transcripts, DCL4 and DCL2 act hierarchically as they do in antiviral resistance to produce 21- and 22-nt siRNAs, respectively, and these guide mRNA degradation. Several dcl genotypes were also tested for their capacity to transmit the mobile silencing signal from the rootstock. dcl1-8 and a dcl2 dcl3 dcl4 triple mutant are compromised in micro-RNA and siRNA biogenesis, respectively, but were unaffected in signal transmission.

Links

PubMed PMC1964546 Online version:10.1073/pnas.0706701104

Keywords

Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Argonaute Proteins; Cell Nucleus/genetics; Cell Nucleus/metabolism; Epigenesis, Genetic; Gene Silencing; Models, Biological; Mutation; RNA Interference; RNA Replicase/genetics; RNA Replicase/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; RNA, Small Interfering/metabolism; Ribonuclease III/genetics; Ribonuclease III/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:RDR2

acts_upstream_of_or_within

GO:0010495: long-distance posttranscriptional gene silencing

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NRPD1

acts_upstream_of_or_within

GO:0010495: long-distance posttranscriptional gene silencing

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AGO4

NOT|involved_in

GO:0010495: long-distance posttranscriptional gene silencing

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RDR2

involved_in

GO:0010495: long-distance posttranscriptional gene silencing

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

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