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PMID:17725544

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Citation

El-Kafafi, el-S, Karamoko, M, Pignot-Paintrand, I, Grunwald, D, Mandaron, P, Lerbs-Mache, S and Falconet, D (2008) Developmentally regulated association of plastid division protein FtsZ1 with thylakoid membranes in Arabidopsis thaliana. Biochem. J. 409:87-94

Abstract

FtsZ is a key protein involved in bacterial and organellar division. Bacteria have only one ftsZ gene, while chlorophytes (higher plants and green alga) have two distinct FtsZ gene families, named FtsZ1 and FtsZ2. This raises the question of why chloroplasts in these organisms need distinct FtsZ proteins to divide. In order to unravel new functions associated with FtsZ proteins, we have identified and characterized an Arabidopsis thaliana FtsZ1 loss-of-function mutant. ftsZ1-knockout mutants are impeded in chloroplast division, and division is restored when FtsZ1 is expressed at a low level. FtsZ1-overexpressing plants show a drastic inhibition of chloroplast division. Chloroplast morphology is altered in ftsZ1, with chloroplasts having abnormalities in the thylakoid membrane network. Overexpression of FtsZ1 also induced defects in thylakoid organization with an increased network of twisting thylakoids and larger grana. We show that FtsZ1, in addition to being present in the stroma, is tightly associated with the thylakoid fraction. This association is developmentally regulated since FtsZ1 is found in the thylakoid fraction of young developing plant leaves but not in mature and old plant leaves. Our results suggest that plastid division protein FtsZ1 may have a function during leaf development in thylakoid organization, thus highlighting new functions for green plastid FtsZ.

Links

PubMed Online version:10.1042/BJ20070543

Keywords

Arabidopsis/genetics; Arabidopsis/physiology; Arabidopsis Proteins/genetics; Chlorophyll/metabolism; Chloroplasts/metabolism; Gene Expression Regulation, Plant; Immunohistochemistry; Microscopy, Confocal; Microscopy, Electron, Transmission; Models, Biological; Organelles/metabolism; Phenotype; Plant Proteins/genetics; Plant Proteins/physiology; Plastids/metabolism; Stem Cells/metabolism; Thylakoids/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:FTSZ1

colocalizes_with

GO:0009535: chloroplast thylakoid membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:FTSZ1

involved_in

GO:0009658: chloroplast organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:FTSZ1

located_in

GO:0009570: chloroplast stroma

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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