GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:17716972

From GONUTS
Jump to: navigation, search
Citation

Liu, L, He, B, Liu, WM, Zhou, D, Cox, JV and Zhang, XA (2007) Tetraspanin CD151 promotes cell migration by regulating integrin trafficking. J. Biol. Chem. 282:31631-42

Abstract

Regulation of cell migration is an important feature of tetraspanin CD151. Although it is well established that CD151 physically associates with integrins, the mechanism by which CD151 regulates integrin-dependent cell migration is basically unknown. Given the fact that CD151 is localized in both the plasma membrane and intracellular vesicles, we found that CD151 and its associated alpha3beta1, alpha5beta1, and alpha6beta1 integrins undergo endocytosis and accumulate in the same intracellular vesicular compartments. CD151 contains a YRSL sequence, a YXXvarphi type of endocytosis/sorting motif, in its C-terminal cytoplasmic domain. Mutation of this motif markedly attenuated CD151 internalization. The loss of CD151 trafficking completely abrogated CD151-promoted cell migration on extracellular matrices such as laminin and diminished the internalization of its associated integrins, indicating a critical role for integrin trafficking in regulating cell motility. In conclusion, the YXXvarphi motif-mediated internalization of CD151 promotes integrin-dependent cell migration by modulating the endocytosis and/or vesicular trafficking of its associated integrins.

Links

PubMed Online version:10.1074/jbc.M701165200

Keywords

Alanine/metabolism; Amino Acid Motifs/genetics; Amino Acid Sequence; Amino Acid Substitution; Animals; Cell Line, Tumor; Cell Movement/physiology; Cytoplasm/chemistry; Endocytosis/physiology; Humans; Integrin alpha3beta1/metabolism; Integrin alpha5beta1/metabolism; Integrin alpha6beta1/metabolism; Integrin alphaV/chemistry; Integrin alphaV/metabolism; Integrins/metabolism; Male; Mice; NIH 3T3 Cells; Prostatic Neoplasms/pathology; Protein Structure, Tertiary; Protein Transport; Subcellular Fractions/metabolism; Transfection

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:CD151

enables

GO:0005178: integrin binding

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:P48509

F

Seeded From UniProt

complete

MOUSE:CD151

acts_upstream_of_or_within

GO:0030335: positive regulation of cell migration

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:P48509

P

Seeded From UniProt

complete

MOUSE:CD151

located_in

GO:0009986: cell surface

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:P48509

C

Seeded From UniProt

complete

MOUSE:CD151

acts_upstream_of_or_within

GO:0045807: positive regulation of endocytosis

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:P48509

P

Seeded From UniProt

complete

MOUSE:CD151

acts_upstream_of_or_within

GO:0044319: wound healing, spreading of cells

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:P48509

P

Seeded From UniProt

complete

MOUSE:ITA5

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P48509

F

Seeded From UniProt

complete

MOUSE:CD44

located_in

GO:0009986: cell surface

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:ITA6

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P48509

F

Seeded From UniProt

complete

MOUSE:TFR1

located_in

GO:0009986: cell surface

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:ITA3

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P48509

F

Seeded From UniProt

complete

MOUSE:EEA1

located_in

GO:0005769: early endosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:RAB5A

located_in

GO:0005769: early endosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:CD44

part_of

GO:0009986: cell surface

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CD151

enables

GO:0005178: integrin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:CD151

involved_in

GO:0045807: positive regulation of endocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CD151

involved_in

GO:0044319: wound healing, spreading of cells

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CD151

involved_in

GO:0045807: positive regulation of endocytosis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CD151

part_of

GO:0009986: cell surface

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CD151

involved_in

GO:0030335: positive regulation of cell migration

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CD151

involved_in

GO:0044319: wound healing, spreading of cells

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CD151

involved_in

GO:0030335: positive regulation of cell migration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:EEA1

part_of

GO:0005769: early endosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

Notes

See also

References

See Help:References for how to manage references in GONUTS.