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PMID:17698500

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Citation

Serrano-Heras, G, Ruiz-Masó, JA, del Solar, G, Espinosa, M, Bravo, A and Salas, M (2007) Protein p56 from the Bacillus subtilis phage phi29 inhibits DNA-binding ability of uracil-DNA glycosylase. Nucleic Acids Res. 35:5393-401

Abstract

Protein p56 (56 amino acids) from the Bacillus subtilis phage 29 inactivates the host uracil-DNA glycosylase (UDG), an enzyme involved in the base excision repair pathway. At present, p56 is the only known example of a UDG inhibitor encoded by a non-uracil containing viral DNA. Using analytical ultracentrifugation methods, we found that protein p56 formed dimers at physiological concentrations. In addition, circular dichroism spectroscopic analyses revealed that protein p56 had a high content of beta-strands (around 40%). To understand the mechanism underlying UDG inhibition by p56, we carried out in vitro experiments using the Escherichia coli UDG enzyme. The highly acidic protein p56 was able to compete with DNA for binding to UDG. Moreover, the interaction between p56 and UDG blocked DNA binding by UDG. We also demonstrated that Ugi, a protein that interacts with the DNA-binding domain of UDG, was able to replace protein p56 previously bound to the UDG enzyme. These results suggest that protein p56 could be a novel naturally occurring DNA mimicry.

Links

PubMed PMC2018632 Online version:10.1093/nar/gkm584

Keywords

Amino Acid Sequence; Bacillus Phages; Bacillus subtilis/virology; Binding, Competitive; DNA/metabolism; Dimerization; Escherichia coli/enzymology; Escherichia coli Proteins/antagonists & inhibitors; Escherichia coli Proteins/metabolism; Molecular Sequence Data; Protein Structure, Secondary; Sequence Alignment; Uracil-DNA Glycosidase/antagonists & inhibitors; Uracil-DNA Glycosidase/metabolism; Urea/chemistry; Viral Proteins/chemistry; Viral Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

BPPH2:P56

GO:0051701

interaction with host

IPI: Inferred from Physical Interaction

UniProtKB:P39615

P

Seeded From UniProt

complete

Notes

See also

References

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