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PMID:17659086

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Citation

Sawai, S, Guan, XJ, Kuspa, A and Cox, EC (2007) High-throughput analysis of spatio-temporal dynamics in Dictyostelium. Genome Biol. 8:R144

Abstract

We demonstrate a time-lapse video approach that allows rapid examination of the spatio-temporal dynamics of Dictyostelium cell populations. Quantitative information was gathered by sampling life histories of more than 2,000 mutant clones from a large mutagenesis collection. Approximately 4% of the clonal lines showed a mutant phenotype at one stage. Many of these could be ordered by clustering into functional groups. The dataset allows one to search and retrieve movies on a gene-by-gene and phenotype-by-phenotype basis.

Links

PubMed PMC2323234 Online version:10.1186/gb-2007-8-7-r144

Keywords

Animals; Dictyostelium/cytology; Dictyostelium/genetics; Dictyostelium/growth & development; Microscopy, Video/methods; Mutation; Phenotype

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

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