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PMID:17646381
| Citation |
Kasahara, K, Ohtsuki, K, Ki, S, Aoyama, K, Takahashi, H, Kobayashi, T, Shirahige, K and Kokubo, T (2007) Assembly of regulatory factors on rRNA and ribosomal protein genes in Saccharomyces cerevisiae. Mol. Cell. Biol. 27:6686-705 |
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| Abstract |
HMO1 is a high-mobility group B protein that plays a role in transcription of genes encoding rRNA and ribosomal proteins (RPGs) in Saccharomyces cerevisiae. This study uses genome-wide chromatin immunoprecipitation to study the roles of HMO1, FHL1, and RAP1 in transcription of these genes as well as other RNA polymerase II-transcribed genes in yeast. The results show that HMO1 associates with the 35S rRNA gene in an RNA polymerase I-dependent manner and that RPG promoters (138 in total) can be classified into several distinct groups based on HMO1 abundance at the promoter and the HMO1 dependence of FHL1 and/or RAP1 binding to the promoter. FHL1, a key regulator of RPGs, binds to most of the HMO1-enriched and transcriptionally HMO1-dependent RPG promoters in an HMO1-dependent manner, whereas it binds to HMO1-limited RPG promoters in an HMO1-independent manner, irrespective of whether they are transcribed in an HMO1-dependent manner. Reporter gene assays indicate that these functional properties are determined by the promoter sequence. |
| Links |
PubMed PMC2099245 Online version:10.1128/MCB.00876-07 |
| Keywords |
DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Forkhead Transcription Factors; Gene Expression Regulation, Fungal; Genome, Fungal; High Mobility Group Proteins/genetics; High Mobility Group Proteins/metabolism; Promoter Regions, Genetic; RNA Polymerase I/metabolism; RNA, Ribosomal/genetics; RNA, Ribosomal/metabolism; Ribosomal Proteins/genetics; Ribosomal Proteins/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Telomere-Binding Proteins/genetics; Telomere-Binding Proteins/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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