GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:17644732

From GONUTS
Jump to: navigation, search
Citation

Chen, Y, Haviernik, P, Bunting, KD and Yang, YC (2007) Cited2 is required for normal hematopoiesis in the murine fetal liver. Blood 110:2889-98

Abstract

Cited2 (cAMP-responsive elementbinding protein [CBP]/p300-interacting transactivators with glutamic acid [E] and aspartic acid [D]-rich tail 2) is a newly identified transcriptional modulator. Knockout of the Cited2 gene results in embryonic lethality with embryos manifesting heart and neural tube defects. Cited2-/- fetal liver displayed significant reduction in the numbers of Lin(-)c-Kit+Sca-1+ cells, Lin(-)c-Kit+ cells, and progenitor cells of different lineages. Fetal liver cells from Cited2-/- embryos gave rise to markedly reduced number of colonies in the colony-forming unit assay. Primary and secondary transplantation studies showed significantly compromised reconstitution of T-lymphoid, B-lymphoid, and myeloid lineages in mice that received a transplant of Cited2-/- fetal liver cells. Competitive reconstitution experiments further showed that fetal liver hematopoietic stem cell (HSC) function is severely impaired due to Cited2 deficiency. Microarray analysis showed decreased expression of Wnt5a and a panel of myeloid molecular markers such as PRTN3, MPO, Neutrophil elastase, Cathepsin G, and Eosinophil peroxidase in Cited2-/- fetal livers. Decreased expression of Bmi-1, Notch1, LEF-1, Mcl-1, and GATA2 was also observed in Cited2-/- Lin(-)c-Kit+ cells. The present study uncovers for the first time a novel role of Cited2 in the maintenance of hematopoietic homeostasis during embryogenesis and thus provides new insights into the molecular regulation of hematopoietic development.

Links

PubMed PMC2018670 Online version:10.1182/blood-2007-01-066316

Keywords

Animals; Bone Marrow Transplantation; Cell Differentiation/physiology; Cell Proliferation; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Fetus; Gene Expression Regulation, Developmental; Hematopoiesis/physiology; Hematopoietic Stem Cells/cytology; Hematopoietic Stem Cells/metabolism; Immunophenotyping; Liver/cytology; Liver/embryology; Liver/metabolism; Mice; Mice, Knockout; Oligonucleotide Array Sequence Analysis; Repressor Proteins/genetics; Repressor Proteins/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Trans-Activators/genetics; Trans-Activators/metabolism; Transduction, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:CITE2

acts_upstream_of_or_within

GO:0002521: leukocyte differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2385355

P

  • occurs_in:(EMAPA:16846)

Seeded From UniProt

complete

MOUSE:CITE2

acts_upstream_of_or_within

GO:0002244: hematopoietic progenitor cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2385355

P

  • occurs_in:(EMAPA:16846)

Seeded From UniProt

complete

MOUSE:CITE2

acts_upstream_of_or_within

GO:0001889: liver development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2385355

P

  • results_in_development_of:(EMAPA:16846)

Seeded From UniProt

complete

MOUSE:CITE2

acts_upstream_of_or_within

GO:0030851: granulocyte differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2385355

P

  • occurs_in:(EMAPA:16846)

Seeded From UniProt

complete

MOUSE:CITE2

acts_upstream_of_or_within

GO:0048821: erythrocyte development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2385355

P

  • occurs_in:(EMAPA:16846)

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.