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PMID:17643380

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Citation

Schneider, C, Anderson, JT and Tollervey, D (2007) The exosome subunit Rrp44 plays a direct role in RNA substrate recognition. Mol. Cell 27:324-31

Abstract

The exosome plays key roles in RNA maturation and surveillance, but it is unclear how target RNAs are identified. We report the functional characterization of the yeast exosome component Rrp44, a member of the RNase II family. Recombinant Rrp44 and the purified TRAMP polyadenylation complex each specifically recognized tRNA(i)(Met) lacking a single m(1)A(58) modification, even in the presence of a large excess of total tRNA. This tRNA is otherwise mature and functional in translation in vivo but is presumably subtly misfolded. Complete degradation of the hypomodified tRNA required both Rrp44 and the poly(A) polymerase activity of TRAMP. The intact exosome lacking only the catalytic activity of Rrp44 failed to degrade tRNA(i)(Met), showing this to be a specific Rrp44 substrate. Recognition of hypomodified tRNA(i)(Met) by Rrp44 is genetically separable from its catalytic activity on other substrates, with the mutations mapping to distinct regions of the protein.

Links

PubMed Online version:10.1016/j.molcel.2007.06.006

Keywords

Amino Acid Sequence; Amino Acid Substitution; Exoribonucleases/chemistry; Exoribonucleases/genetics; Exoribonucleases/metabolism; Fungal Proteins/chemistry; Fungal Proteins/genetics; Fungal Proteins/metabolism; Molecular Sequence Data; Mutagenesis, Site-Directed; RNA Processing, Post-Transcriptional; RNA, Fungal/genetics; RNA, Fungal/metabolism; RNA, Transfer, Met/genetics; RNA, Transfer, Met/metabolism; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Sequence Homology, Amino Acid; Serine Endopeptidases/metabolism; Substrate Specificity

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

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