GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:17576511

From GONUTS
Jump to: navigation, search
Citation

Jan, C, Muriel, MP, Rolland, AS, Hirsch, EC and François, C (2007) Localization of D1a dopamine receptors on cell bodies and axonal endings in the substantia nigra pars reticulata of the rat. J Neural Transm (Vienna) 114:1509-17

Abstract

In the present study, we analyzed the localization of D1a receptors within the rat substantia nigra pars reticulata (SNr) using specific D1a immunochemistry at the ultrastructural level and RT-PCR. At the electron microscopic level, D1a receptors were strongly associated with axons and axonal endings in the SNr, but also with numerous glutamic acid decarboxylase-positive dendrites and neuronal cell bodies. This neuronal expression of D1a receptors was confirmed using RT-PCR. G(alphaolf) protein-specific immunostaining displayed a similar distribution in dendrites and cell bodies to that of D1a receptors. The localization of D1a receptors in both GABAergic cell bodies and terminals is in accordance with the well known complex action of dopamine in the SNr. Moreover, the intracytoplasmic localization of D1a receptors in cell bodies and dendrites that we observed suggests that these receptors are only effective in specific conditions, or are transported to different nigral targets where they may have a presynaptic function.

Links

PubMed Online version:10.1007/s00702-007-0775-8

Keywords

Animals; Dendrites/metabolism; Dendrites/ultrastructure; Immunoblotting; Immunohistochemistry; Male; Microscopy, Electron, Transmission; Nerve Endings/metabolism; Nerve Endings/ultrastructure; Neurons/metabolism; Neurons/ultrastructure; RNA, Messenger/analysis; Rats; Rats, Sprague-Dawley; Receptors, Dopamine/biosynthesis; Receptors, Dopamine/ultrastructure; Reverse Transcriptase Polymerase Chain Reaction; Substantia Nigra/metabolism; Substantia Nigra/ultrastructure

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:DRD1

located_in

GO:0043679: axon terminus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:DRD1

located_in

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:DRD1

located_in

GO:0030424: axon

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:DRD1

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:DRD1

located_in

GO:0043025: neuronal cell body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:DRD1

part_of

GO:0030424: axon

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:DRD1

part_of

GO:0043025: neuronal cell body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:DRD1

part_of

GO:0043679: axon terminus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:DRD1

part_of

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:DRD1

part_of

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

Notes

See also

References

See Help:References for how to manage references in GONUTS.