PMID:17565989

Citation	Iwasaki, K, Hailemariam, K and Tsuji, Y (2007) PIAS3 interacts with ATF1 and regulates the human ferritin H gene through an antioxidant-responsive element. J. Biol. Chem. 282:22335-43
Abstract	Gene transcription is coordinately regulated by the balance between activation and repression mechanisms in response to various external stimuli. Ferritin, composed of H and L subunits, is the major intracellular iron storage protein involved in iron homeostasis. We previously identified an enhancer, termed antioxidant-responsive element (ARE), in the human ferritin H gene and its respective transcriptional activators including Nrf2 and JunD. Here we found that ATF1 (activating transcription factor 1) is a transcriptional repressor of the ferritin H ARE. Subsequent yeast two-hybrid screening identified PIAS3 (protein inhibitor of activated STAT3) as an ATF1-binding protein. Further investigation of the human ferritin H ARE regulation showed that 1) PIAS3 reversed ATF1-mediated repression of the ferritin H ARE; 2) ATF1 was sumoylated, but PIAS3, a SUMO E3 ligase, did not appear to play a major role in SUMO1-mediated ATF1 sumoylation or ATF1 transcription activating function; 3) PIAS3 decreased ATF1 binding to the ARE; and 4) ATF1 knockdown with siRNA increased ferritin H expression, whereas PIAS3 knockdown decreased basal expression and oxidative stress-mediated induction of ferritin H. These results suggest that PIAS3 antagonizes the repressor function of ATF1, at least in part by blocking its DNA binding, and ultimately activates the ARE. Collectively our results suggest that PIAS3 is a new regulator of ATF1 that regulates the ARE-mediated transcription of the ferritin H gene.
Links	PubMed PMC2409283 Online version:10.1074/jbc.M701477200
Keywords	Activating Transcription Factor 1/metabolism; Animals; Antioxidants/metabolism; Apoferritins/genetics; DNA-Binding Proteins/metabolism; Humans; K562 Cells; Mice; Molecular Chaperones/metabolism; NF-E2-Related Factor 2/metabolism; NIH 3T3 Cells; Nuclear Proteins/metabolism; Protein Binding; Protein Inhibitors of Activated STAT/metabolism; RNA, Small Interfering/metabolism; SUMO-1 Protein; Small Ubiquitin-Related Modifier Proteins/metabolism; Transcription Factors
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:ATF1		GO:0035326 : enhancer binding	ECO:0000314:		F		figure 1	complete
HUMAN:ATF1		GO:0016481 : negative regulation of transcription	ECO:0000315:		P		Fig.2B,C	complete
HUMAN:ATF1		GO:0016925 : protein sumoylation	ECO:0000314:		P		Fig. S1	complete
HUMAN:PIAS3		GO:0005515 : protein binding	ECO:0000021:	UniProtKB:P18846	F		Figure.3	complete
HUMAN:PIAS3		GO:0045449 : regulation of transcription	ECO:0000315:		P		figure 4	complete
HUMAN:PIAS3		GO:0010628 : positive regulation of gene expression	ECO:0000315:		P		figure 7	complete

References

See Help:References for how to manage references in GONUTS.