GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:17563371

From GONUTS
Jump to: navigation, search
Citation

Ren, JG, Li, Z and Sacks, DB (2007) IQGAP1 modulates activation of B-Raf. Proc. Natl. Acad. Sci. U.S.A. 104:10465-9

Abstract

IQGAP1 modulates several cellular functions, including cell-cell adhesion, transcription, cytoskeletal architecture, and selected signaling pathways. We previously documented that IQGAP1 binds ERK and MAPK kinase (MEK) and regulates EGF-stimulated MEK and ERK activity. Here we characterize the interaction between IQGAP1 and B-Raf, the molecule immediately upstream of MEK in the Ras/MAPK signaling cascade. B-Raf binds directly to IQGAP1 in vitro and coimmunoprecipitates with IQGAP1 from cell lysates. Importantly, IQGAP1 modulates B-Raf function. EGF is unable to stimulate B-Raf activity in IQGAP1-null cells and in cells transfected with an IQGAP1 mutant construct that is unable to bind B-Raf. Interestingly, binding to IQGAP1 significantly enhances B-Raf activity in vitro. Our data identify a previously unrecognized interaction between IQGAP1 and B-Raf and suggest that IQGAP1 is a scaffold necessary for activation of B-Raf by EGF.

Links

PubMed PMC1965536 Online version:10.1073/pnas.0611308104

Keywords

Animals; Cell Line, Transformed; Cells, Cultured; Culture Media, Serum-Free; Embryo, Mammalian; Enzyme Activation; Epidermal Growth Factor/pharmacology; Fibroblasts/cytology; Fibroblasts/metabolism; Glutathione Transferase/metabolism; Humans; Mice; Mice, Knockout; Mutation; Plasmids/metabolism; Precipitin Tests; Protein Binding; Protein Structure, Tertiary; Proto-Oncogene Proteins B-raf/analysis; Proto-Oncogene Proteins B-raf/metabolism; Recombinant Fusion Proteins/metabolism; Time Factors; Transfection; ras GTPase-Activating Proteins/chemistry; ras GTPase-Activating Proteins/genetics; ras GTPase-Activating Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:BRAF

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P46940

F

Seeded From UniProt

complete

MOUSE:BRAF

enables

GO:0004672: protein kinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:BRAF

involved_in

GO:0006468: protein phosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:IQGA1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P15056

F

Seeded From UniProt

complete

HUMAN:IQGA1

enables

GO:0043539: protein serine/threonine kinase activator activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:IQGA1

involved_in

GO:0045860: positive regulation of protein kinase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:IQGA1

involved_in

GO:0045860: positive regulation of protein kinase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:IQGA1

enables

GO:0043539: protein serine/threonine kinase activator activity

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_direct_input:(UniProtKB:P15056)
  • part_of:(GO:0071364)|has_direct_input(UniProtKB:P15056)
  • part_of:(GO:0007173)

Seeded From UniProt

complete

HUMAN:IQGA1

involved_in

GO:0071364: cellular response to epidermal growth factor stimulus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:IQGA1

involved_in

GO:0007173: epidermal growth factor receptor signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:IQGA1

involved_in

GO:0045860: positive regulation of protein kinase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • has_regulation_target:(UniProtKB:P15056)
  • part_of:(GO:0007173)|has_regulation_target(UniProtKB:P15056)
  • part_of:(GO:0071364)

Seeded From UniProt

complete

HUMAN:IQGA1

enables

GO:0019901: protein kinase binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P15056

F

Seeded From UniProt

complete

HUMAN:BRAF

involved_in

GO:0006468: protein phosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:BRAF

enables

GO:0004672: protein kinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:IQGA1

involved_in

GO:0045860: positive regulation of protein kinase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • has_regulation_target:(UniProtKB:P28028)
  • part_of:(GO:0007173)|has_regulation_target(UniProtKB:P15056)
  • part_of:(GO:0071364)

Seeded From UniProt

complete

MOUSE:IQGA1

involved_in

GO:0007173: epidermal growth factor receptor signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:IQGA1

involved_in

GO:0071364: cellular response to epidermal growth factor stimulus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:IQGA1

involved_in

GO:0036120: cellular response to platelet-derived growth factor stimulus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:IQGA1

involved_in

GO:0048008: platelet-derived growth factor receptor signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:IQGA1

involved_in

GO:0008543: fibroblast growth factor receptor signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:IQGA1

involved_in

GO:0044344: cellular response to fibroblast growth factor stimulus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.