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PMID:17557114

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Citation

Cvetanovic, M, Rooney, RJ, Garcia, JJ, Toporovskaya, N, Zoghbi, HY and Opal, P (2007) The role of LANP and ataxin 1 in E4F-mediated transcriptional repression. EMBO Rep. 8:671-7

Abstract

The leucine-rich acidic nuclear protein (LANP) belongs to the INHAT family of corepressors that inhibits histone acetyltransferases. The mechanism by which LANP restricts its repression to specific genes is unknown. Here, we report that LANP forms a complex with transcriptional repressor E4F and modulates its activity. As LANP interacts with ataxin 1--a protein mutated in the neurodegenerative disease spinocerebellar ataxia type 1 (SCA1)--we tested whether ataxin 1 can alter the E4F-LANP interaction. We show that ataxin 1 relieves the transcriptional repression induced by the LANP-E4F complex by competing with E4F for LANP. These results provide the first functional link, to our knowledge, between LANP and ataxin 1, and indicate a potential mechanism for the transcriptional aberrations observed in SCA1.

Links

PubMed PMC1905893 Online version:10.1038/sj.embor.7400983

Keywords

Animals; Cell Line; Cell Line, Tumor; Cercopithecus aethiops; Chromatin Immunoprecipitation; Genes, Reporter; HeLa Cells; Humans; Immunohistochemistry; Luciferases/metabolism; Mice; Mutation; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Neuroblastoma/pathology; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Phosphoproteins/genetics; Phosphoproteins/metabolism; Precipitin Tests; Repressor Proteins/metabolism; Spinocerebellar Ataxias/metabolism; Two-Hybrid System Techniques

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:AN32A

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q8CCE9

F

Seeded From UniProt

complete

MOUSE:AN32A

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P54253

F

Seeded From UniProt

complete

HUMAN:AN32A

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q66K89

F

Seeded From UniProt

complete

HUMAN:ATX1

located_in

GO:0016363: nuclear matrix

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:ATX1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O35381

F

Seeded From UniProt

complete

HUMAN:E4F1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P39687

F

Seeded From UniProt

complete

MOUSE:E4F1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O35381

F

Seeded From UniProt

complete

HUMAN:ATX1

part_of

GO:0016363: nuclear matrix

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

See also

References

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